104 Wisconsin Academy of Sciences, Arts, and Letters. 



in diameter with a narrow dark margin. In these spots the leaf 

 parenchyma separates from the venules and probably falls away 

 leaving the venular network. The zonate spots look much like 

 the work of leaf miners, the dark lines suggesting burrows con- 

 taining excreta. From the closely massed, erect, straight hyphae 

 of the acervuli are abstricted hyaline filiform sporules 7-11 x 2/*. 

 Fungi Columbiani 1587 (on Populus trcmuloides, Newfield, N. 

 J. J. B. Ellis.) issued under the name Septoria musiva Pk. 

 bears similar but somewhat larger spots (1-2 cm.) and sporules 

 18-30x2-3/* uniseptate. I am considering the Wisconsin collection 

 to be a microconidial state of this which I refer to Marssonina 

 rhabdospora E. & E. 



Since this was written collections on Populus grandidentata 

 have been made at Phlox and Neopit. The following notes were 

 made from the latter: Spots circular, alutaceous shading out- 

 ward into reddish brown and with a darker margin, the upper 

 surface darker than the lower, 1-4 mm. in diameter, sometimes 

 confluent; acervuli hypophyllous, usually few; sporules gener- 

 ally straight, uniseptate, 18-33 x 2-3/*. In the Phlox collection 

 the spots are more numerous, rather more angular and more 

 frequently confluent, 



Cylindrosporium vermiforme n. sp. Spots amphigenous, sub- 

 circular to irregular, immarginate, brown, 5-15 mm. in diam- 

 eter; acervuli epiphyllous, scattered, subcuticular, flat, 40-60/* in 

 diameter; sporules. hyaline, vermiform, curved, sigmoid or 

 flexuose, pluriseptate, 150-250x4-5/*. On leaves of Alnus in- 

 cana Devils Lake. Wisconsin. The sporules suggest eel worms 

 in appearance. Specimens in the herbarium of the Uni- 

 versity of Wisconsin collected at Devils Lake, August 15th, 

 1906, by Pi. A. Harper show many of the sporules pro- 

 vided with a rostrum, 10-42x1-1%/*, at the apex. A collec- 

 tion made in August, 1913, does not show the "rostrum" 

 but one made September 1, 1913, showed some of the spor- 

 ules so provided. Living sporules, germinating in water, in 

 addition to the lateral germ tubes, put forth one from the apex 

 so like the "rostrum" that I infer that the beak is a germ 

 tube put forth while the sporule is still in situ. Some spor- 

 ules bearing a beak put forth in water a second tube alongside 

 the rostrum and similar to it. Because of the large, erumpent, 

 fasciculate sporules this might be referred to Hyphal< s. 



