28 The Ohio Journal of Science [Vol. XYI, No. 1, 



sequitur) inflatis, altero rectis; tubo conjugationis plerumque 

 ex cellula mascula emisso; zygosporis ellipticis 37-42/i x 57-100)U 

 membrana media micropunctata et lutea. 



Vegetative cells 30-30^x120-300^, end walls plane; 1 

 chromatophore making 3-7 turns; fertile cells scattered in twos 

 or fours among vegetative cells, or continuous, inflated on the 

 conjugating side, outer side straight; conjugating tubes formed 

 almost wholly by the male cell, zygospores ellipsoid 37-42// x 

 57-70/i, median wall minutely punctate, yellow. Type in herb. 

 E. N. T. Coll. No. 2470, 2953. 



This species was first found in the West Big Four Pond, 

 east of Charleston, Illinois. It has since been found in a col- 

 lection from Chocorua, N. H., sent me by Mr. Chas. Bullard. 

 It evidently belongs in the punctata group of the Spirogyras, 

 but in form and markings of the spore, and the shape of the 

 fertile cells it is amply distinct from its nearest allies; 6*. 

 punctijormis Transeau and the next species to be described. 



S. reflexa nov. sp. 



Cellulis vegetativis 30-40/x x 120-300/1, dissepimentis planis; 

 chromatophoris singulis anfractibus arctis 3-8/x cellulis fructiferis 

 binis vel quaternis inter cellulas vegetativas distributis. inflatis 

 et valde reflexis; tubo conjugationis ex cellula mascula emisso; 

 zygosporis ellipticis, 44-54/i x 90-150//, membrana media glabra 

 et luteo-brunnea. 



Vegetative cells 30-40/x x 120-300/x, with plane end wall; 1 

 chromatophore making 3-8 turns; fertile cells in groups of 2 or 4, 

 inflated or enlarged and strongly reflexed; conjugating tube 

 formed by the male cells; zygospores ellipsoid, 44-54/1 x 90-150/i, 

 median wall smooth, yellow-brown. Type in herb. E. N. T. 

 Collection No. 2661, 2664, 2912. 



This species has been under observation for four years and 

 has been collected from ponds near Casey, Lerna, Coffeen and 

 Donnellson, Illinois. The large, smooth spores, the reflexed 

 conjugating cells, and the tube produced wholly by the male 

 cells are the distinguishing characteristics. 



S. hydrodictya nov. sp. 



Cellulis vegetativis 75-100/i x 210-360/x, dissepimentis planis, 

 chromatophoris 7-10, modo subrectis longitudinalibus, modo 

 spiralibus anfractibus arctis .1-.5; celluHs fructiferis inflatis vel 



