March, 1907.] The Public Drinking Cup. 107 



given a drink from the cup. The swab was taken directly 

 afterward. The cover glass preparation showed some bacteria 

 which we were not able to cultivate. The following bacteria 

 were shown to be present: 



1. Sarcina lutea (Fltigge). (See above). 



2. Micrococcus liquifacians (v. Besser). First isolated from 

 nasal mucous. 



3. Micrococcus pyogenes aureus (Rosenbach) Pathogenic 

 (pyogenic). Common. 



4. Micrococcus pyogenes albus (Rosenbach). Pathogenic 

 (pyogenic). Common. 



5. Pseudomonas fluorescens var. liq. (Fliigge). (See above.) 



6. Bacillus vulgatus (Trevisan). Widely distributed. 



7. Bacterium bucallis. Isolated first from healthy sputum. 

 Ex. No. 3. Swab was taken from a cup in connection with an 



improvised water tap and was used by several hundred working- 

 men. The cup remained in the dirt and mud underneath the tap 

 the most of the time. The following bacteria were shown to be 

 present. 



1. Sarcina aurantica (Fliigge). Widely distributed in air and 

 water. 



2. Micrococcus roseus (Bumm). Widely distributed in air. 



3. Micrococcus pygoenes albus (Rosenbach). (See above.) 



4. Streptococcus pyogenes aureus (Rosenbach) St. erysipela- 

 tis (Fehleisen) . A virulent pyogenic bacterium which may cause a 

 variety of disease conditions. 



5. Pseudomonas janthina (Zopf). Distributed in water. 



6. Spirillum rubrum (v. Esmarch). Distributed in water. 



An effort was made to trace any cases of streptococcus infec- 

 tion which might have resulted from this cup but owing to the 

 difficulty of getting at the workingmen this could not be done. 

 It should be noted in this case that there are no soil bacteria 

 present notwithstanding the fact that the cup was frequently 

 contaminated. No anaerobes could be demonstrated in this case. 



Ex. No. 4. Swab was taken from a cup in connection with a 

 public drinking fountain in Chicago. The following bacteria 

 were shown to be present: 



1. Sarcina lutea (Fliigge). (See above.) 



2. Sarcina aurantica (Fliigge). (See above.) 



3. Micrococcus pyogenes albus (Rosenbach). (See above.) 



4. Bacillus formosus (Ravenel). Distributed in water. 



5. Pseudomonas fluorescens, var. liq. (Fliigge). (See above). 



6. Bacterium mycoides (Fliigge). Distributed in soil and 

 water. 



The primary cover glass preparation showed a leptothrix 

 which could not be cultivated. This bacterium was in all prob- 

 ability Leptothrix bucallis maxima, (Miller). 



