412 The Ohio Naturalist. [Vol. IX, No. 2, 



record to the contrary with reference to the eggs of xA.sterias 

 Forbesii. With the exception of the stronger solution of 

 strychnine, Phaseolus was able to live in concentrations which 

 would prove fatal to animals. 



The effect of the action of these alkaloids upon plants varies 

 both with the nature and with the strength of the solution, but on 

 the whole a general rise and intensification of the vital processes 

 is seen as, for instance, in the augmentation of the function of 

 transpiration, and in the higher rate of regeneration as compared 

 with plants not subjected to the influence of stimulating agents. 

 It seems therefore, legitimate to assume that there exists an 

 intimate relation between the rate of regeneration and the 

 physiological condition of the regenerating organism. 



Concerning the rate of regeneration after consecutive opera- 

 tion it is obvious, from the facts cited above, that after the second 

 operation there is a considerable decrease of the power of regen- 

 eration and, consequently, a slowing down of the process, as well 

 as a considerable lengthening of the period which intervenes 

 between the operation and the first appearance of regenerated 

 tissue. 



The work here recorded was carried on in the Botanical Lab- 

 oratorv of the Ohio State University during the Spring of 1908, 

 with the aid of a grant from the McMillin Research Fund. 



It gives me pleasure to express here my gratitude to Dr. A. 

 Dachnowski for much friendly assistance in this work. 



Cambridge, Mass., October, 190S. 



LITERATURE. 



1. Dachnowski, A. — The toxic property of bog water and 

 bog soil. Bot. Gaz. 46: 130-144, 1908. 



2. GoEBEL, W. — Regeneration in Plants. Bull. Torrey Bot. 

 Club. 30: 197-205, 1903. 



3. Livingston, B. E. — Relation of transpiration to growth 

 in wheat. Bot. Gaz. 40: 178, 1905. 



4. McCallum, W. B. — Regeneration in Plants. Bot. Gaz. 

 40: 241, 1905. 



5a. MoRGULis, S. — Observations and experiments on regen- 

 eration in Lumbriculus. Journ. Exp. Zool. 4: 549-574, 1907. 



5b. The effect of alkaloids on the early development of 

 Toxopnenstes variegatus. Proc. Amer. Acad. Arts and Sciences, 

 Vol. 44, No. 5, pp. 131-146, 1908. 



6. Pfeffer, W. — Physiology of Plants, Vol. II. 



7. Yasuda, A. — On the elTect of alkaloids upon some moulds. 

 The Bot. Mag., Tokyo, 15: 79-83, 1901. 



8. Zeleny, C. — The efifect of degree of injury, successive 

 injury and functional activity upon regeneration in the Scypho- 

 zoon Cassiopea xamachana. Journ. Exp. Zool. 5: 265-274, 1907. 



