662 THE XODLLE OliGANLSM OF THK LEUU.MINOS.K, 



pouring these into Petri dishes. In about 10 days colonies grew 

 on one of the plates to a millimetre in diameter. Different media 

 inoculated from one of the colonies showed the following cliarac- 

 tei'istics when grown at 22"^ C. : — 



Meat-yelafine plate. — The surface colonies appeal' as raised hemi- 

 spheres with a white, glistening, paraffin-like appearance; 

 glutinous when touched with the needle. With 60-fold 

 magnification they are circular and opaque except at the 

 margins where a little liglit passes through showing a 

 granular structure. The deep colonies are oval or round, 

 brownish and coarsely granular. 



Stah ciiltnref! in. trirunis yelatine media. — White uncharacteristic 

 growth along the needle track; slight surface growth. 



Lupin-ac/d)- 7vith i% j^otasnium chloride. — Luxuriant, stearine-like 

 growth which has extracted some of the colour of the medium. 



Meat-agar sfi'oke. — The inoculating loop has produced a thin, 

 rough, glistening, whitish ribbon with rough margins; the 

 culture gravitates slightly to lowei- portions; growth ne\er 

 luxuriant. 



GJ i/cerine-meat-agar stroke. — At first the growth is like that on 

 meat-agar, later it becomes more luxurious. In three weeks 

 there is an exceedingly voluminous raised, spreading, white 

 glistening culture. 



Inorganic fluid media. — Scanty growth. 



Peptone-ghicose fluid media. — Turbid with slight film and floccu- 

 lent precipitate. 



Peptone-sucrose fluid media. — Clear with film and precijjitate 

 chiefly of old films. 



Potato, ordinary acid. — A yellowish-white, spreading, glistening 

 layer. 



Liiqyvn-e.vfract, etc., gelatine plate — Translucent, white, raised, non- 

 spreading colonies. With 60-fold magnification, circular 

 granular colonies with sharp margin; the deep colonies are like 

 the surface ones, but are more opaque, and consequently 

 appeal' more gi'anular. 



