-f2 The Ohio Naturalist. [Vol. XIII, No. 1, 



-observed in Hemerocallis by Fulmer (4). In some cases the 

 cytoplasm between two newly formed nuclei was separated 

 although no visible wall was formed; but in most cases the nuclei 

 were formed and remained imbedded in the general cytoplasm 

 (Figs. 28-34). In one case a regular tripartite arrangement of 

 three nuclei was observed and these were surrounded by separate 

 masses of cytoplasm (Fig. 31). In none of these cases was the 

 original sporocyte wall disintegrated. The further development 

 of the smaller nuclei was not studied although that might be an 

 interesting investigation. 



SUMMARY. 



1. In the reduction division of Fuchsia speciosa there is 

 apparently an end to end fusion of the univalent chromosomes, 

 forming a continuous spirem which twists and folds up into a 

 definite nmnber of loops which represent the incipient bivalent 

 chromosomes, fourteen in number. 



2. The loose network of the resting nucleus at an early stage 

 begins to show a massing of chromatin material into indefinite 

 lumps of approximately the reduced number of chromosomes. 

 These masses probably represent the arrangement of the chromatin 

 into a definite mosaic, preparatory to the synaptic conjugation of 

 the univalent into the bivalent chromosomes. 



3. Gradually the lumps disappear and the material seems to 

 go toward the formation of prominent granules that arrange 

 themselves along a delicate thread. 



4. Next follows a period during which there is an evident 

 swelling of the nucleus. In consequence of this swelling the threads 

 are pulled loose or withdrawn from the nuclear wall, and the chroma- 

 tin material collapses in a mass. It may collapse around the nucleolus 

 or to one side of it, or it may collapse so that the nucleolus has no 

 connection with it. The contracted portion may lie in the center 

 of the nucleus or in contact with the nuclear wall. This synizesis 

 is regarded as an artifact although no definite evidence was 

 obtained for or against this supposition. 



5. After the synizesis the spirem is apparently continuous and 

 the granules appear small and evenly distributed throughout its 

 length. At first there is little or no looping but soon the spirem 

 begins to show that it is laid in delicate little loops. The loops 

 are arranged on the inside of the nuclear wall. In some sporo- 

 cytes as high as eight loops could be deteniiined at a rather early 

 stage, still more or less connected, but by the time the fourteen 

 loops are fully fomied they are usually broken apart. 



6.' After breaking apart the loops thicken and tighten until 

 masses of various sizes and shapes were formed, fotu" being quite 

 small and five of rather large size. 



