BV H. G. CHAPMAN. 



537 



These maximal precipitates were obtained by mixing 0'02 gm. 

 <lriecl antiserum and 0-004 gm. dried homologous protein. The 

 reaction was allowed to take place in a pipette graduated to 



Table vi. 



hundredths of a cubic centimetre. The volume of the precipitate 

 was read in 48 hours. On comparing Tables vi. and vii., it is seen 

 that there is no correspondence between the amount of protein 



Table vii. 



Antiserum. 



Hen egg 1 



Hen egg 2 

 Hen egg 3 



Ostrich egg 

 Horse serum 1 

 Horse serum 2 

 Dasyure serum 



Volume of precipitum from 0-0 

 dried antiserum. 



0-024 c.c. 

 0-021 c.c. 

 0-010 c.c. 

 0-10 c.c. 

 0-0-24 c c. 

 0-OOSc.c. 

 0-0025 c.c. 



necessary to neutralise the precipitin in an antiserum and the 

 amount of precipitate to be obtained from an antiserum on 

 neutralising the precipitin completely. 



Other series of experiments have been carried out in which the 

 amount of liomologous protein was constant and the amount of 

 antiserum was varied. Such a series is recorded in Table viii. 



42 



