204 BACTERIOLOGICAL OBSERVATIONS MADE AT LITTLE BAY, 



my best thanks. Dui'ing the time of my residence in the above 

 Hospital, Dr. W. Peirce, Medical Superintendent, and Dr. R. "W. 

 Young, Resident Medical Officer, rendered me every possible 

 assistance ; I have much pleasure in gratefully acknowledging 

 this once more. 



My endeavours to cultivate the typhoid-bacilli extended, in the 

 main, to organs of persons who died of typhoid fever, and to 

 dejections from typhoid fever patients. 



It is well to say in advance that in order to get and to isolate 

 the micro-parasite in question, I made use both of Koch's plate- 

 process, and Esmarch's method of cultivating in test-tubes. About 

 the former nothing more requires to be mentioned. Of the latter 

 which I took occasion to demonstrate at the last meeting of this. 

 Society, April, 1887, a detailed description by Esmarch is given in 

 Zeitschrift filr Hygiene, Band I., Heft 2, Leipzig, 1886, pp. 293- 

 301 ; an abstract in Fliigge's Microorganismen, Leipzig, 1886, 

 p. 656. This method was given the preference later on, because 

 the working after the same is connected with less consumption of 

 time, does not require much apparatus, and if properly done 

 yields quite good results. According to my experience very 

 satisfactory cultivations after this process can be obtained in the 

 following mannei". The test-tubes containing the liquefied inocu- 

 lated gelatine (or agar-agar), which in the commonly used test- 

 tubes should not be more than about 6 ccm. — I always employed an 

 8 p.c. gelatine — are first supplied at the top as well as the bottom,, 

 with tightly fitting caps of indiarubber. (Thus the tubes when 

 laid on a level surface are also nearly level). The solidification of 

 the still liquid gelatine (or agar-agar) takes place by means of iced 

 water or at least sufficiently cold water in an ordinary soup-plate, 

 to the bottom of which the tube in its length is loosely pressed by 

 one hand, and quickly turned round its long axis by the other. In 

 a very short time the inner walls of the test-tube show a thin equal 



