OF PUCCINIA CORONATA, VAR. HIMALENSIS. 229 
uredospores occurred throughout the year (an improbable hypothesis) or that the 
mycelium persisted in the root, though I could not detect any hyphs by microscopic 
examination. Early in the spring of this year (1890) І uprooted a plant with dried 
leaves bearing numerous teleutospore pustules and kept it in my laboratory with its 
roots in water, changing the water frequently. It unfolded new green leaves shortly 
afterwards, and on the 17th of May I found several uredo pustules on one of them, 
proving almost conclusively that the mycelium is perennial In the case of Brachy- 
podium I do not think the same condition exists, as it is rare to find uredospores оп it 
until the zecidia of Rhamnus are ripe. I did, however, one year find in one locality, in 
nature, several leaves of what I thought was Brachypodium bearing uredospores as early 
as the 29th of March. It is possible, however, that I was mistaken in my identification 
of the host, a somewhat difficult matter in Grasses before flowering ; but the spores were 
fairly characteristic. The only other explanation I can offer is that they were produced 
by uredospores from Piptatherum. Had the mycelium been perennial in Brachypodium, 
I must have observed uredo pustules on the leaves as frequently as I did on Piptatherum. 
I would here also draw attention to another remarkable peculiarity of this fungus, and 
that is the long time during which the uredospores of Piptatherum and Festuca retain 
vitality and capability of germination under favourable conditions. I collected some 
leaves, which were just beginning to wither, on the 16th of January, and these contained 
numerous teleutospore pustules, but also a few uredo pustules. These leaves were cut 
up into small pieces, and kept in a glass beaker covered over loosely with a large 
wateh-glass. On the 2nd June, i. е. 4} months later, I scraped off spores and floated 
them on water and kept them in a moist atmosphere. After 24 hours the uredospores 
had germinated most freely, although the teleutospores had not. I shall recur to this 
subject later; meanwhile I proceed to a systematic description of the fungus. 
I. HCIDIUM. 
а. Spermogonia.—These are not very numerous, and precede the zecidia by a consider- 
able interval; they are formed on already highly-hypertrophied parts, in which the 
mycelial filaments contain orange-red oil-globules. The normal thickness of the leaf- 
blade being 0-144 mm., the parts bearing spermogonia are about 0:550. Тһе spermogonia 
are both epi- and hypophyllous, but oftener epiphyllous. They are deeply set, measure 
about 0:107 mm. in depth and breadth, and have tufts of protruding paraphyses. 
b. Peridium.—The peridial tube in dry weather is very long and cylindrical, measuring 
about 2 mm. in length by 0:5 mm. in diameter, and opening at the summit by frayed 
and everted edges. It consists of a single layer of cells, angular (4- to 6-sided), 
measuring 26 x 16 p, containing orange-red oil-globules in the centre, and spiny. Тһе 
peridia are hypophyllous and usually on a deeply concave surface. 
с. ZEcidiospores.—These are bright orange-red, round to oval, beset densely with fine 
tubercles, measuring about 23 w in diameter when fresh and just wetted, but 31-19 х 
24-18 p, after being 24 hours іп a moist atmosphere. They germinate readily in water, 
throwing out long, simple, unbranched tubes, into the distal ends of which wander the 
2x2 
