280 ; MR. A. BARCLAY ON THE LIFE-HISTORY 
coloured spore-contents, leaving the spore-walls colourless or grey. The emptied spores 
are then seen, especially with staining, to possess six germ-pores. 
II. UREDO. 
A. On Brachypodium sylvaticum.—These pustules are somewhat larger than the 
teleutospore pustules as a rule. They are orange-red, circular, minute, isolated, very 
numerous, and epiphyllous. The spores are pale saffron-yellow or orange-yellow bodies 
(the coloured contents aggregated in the centre of the spore), round to oval, measuring 
24-20 x 21-15 №. "The epispore is very finely tuberculated, and is piereed by 3 to 4 
germ-pores. They germinate in the usual way, throwing out a single simple tube. 
B. On Piptatherum holciforme and Festuca gigantea.—The pustules are shortly linear 
to round, orange-yellow, mostly epiphyllous, and several may often be seen on one 
discoloured patch extending right across the blade. The spores are round to oval, pale 
yellow or orange-yellow, with the coloured contents aggregated in the centre, echinulate, 
and measuring, when fresh, 28-22 x 22—90 и. These uredospores are therefore consider- 
ably larger than those from Brachypodium. Each spore has 4 to 6 germ-pores. They 
germinate in the usual way, throwing out a single unbranched tube, in the distal end 
of which the coloured contents accumulate, leaving the spore-walls colourless. 
III. TELEUTOSPORE. 
A. On Brachypodium sylvaticum.—The pustules are minute black points on the upper 
leaf-surface and quite naked (Pl. LVI. fig. 8) The spores are brown, with the 
characteristie crown of usually regular processes, borne on short stalks. Тһе spores 
narrow gradually towards the stalk, and are scarcely, if at all, constricted at the septum 
(Pl LVI. figs. 8 and 9). They have a smooth epispore, and measure when fresh 
44-38 х 11-10 и (at the septum). They germinate only after a winter rest, and I have 
observed this from the end of March to August. "The promycelium from the upper cell 
emergés from a point immediately under the crown, and that from the lower cell from 
near the septum. Four sporidia are usually formed by each at the ends of short 
pointed sterigmata. "The sporidia are oval, and measure 18—10 x 9-6 м (Pl. LVI. fig. 11). 
B. On Piptatherum holciforme and Festuca gigantea.—The pustules are black, more 
or less completely covered by epidermis, linear, and hypophyllous (Pl. LVI. fig. 2). 
The spores are brown, crowned with processes, which are, however, generally more 
irregular than those above described, and borne on short stalks. Тһе crown processes 
usually number five, but vary from one to six or seven (РІ. LVI. fig. 4). They do 
not narrow so regularly towards the stalk, and spores may frequently be found rounded 
in both cells and constrieted at the septum (Pl. LVI. figs. 4 and 13). "They are 
smooth externally, and measure 55-48 X14-8 и (septum). They germinate after а 
Shorter rest, as I have seen them growing in my laboratory as early as the 15th 
February, when most other teleutospores still refused to do во. After germination the 
spore-walls remain ehestnut-brown in colour. Four sporidia are formed by each pro- 
mycelium, measuring 9X 7 и. They are pale orange-yellow and often form secondary 
