ОҒ PUCCINIA CORONATA, VAR. HIMALENSIS. 231 
EE 
sporidia. Тһе fungus on this host is not unlike that described by Lagerheim as 
P. gibberosa *. Мог apparently is it unlike Plowright's Puccinia Festuce in general 
characters ; but as the only account of this fungus which I have seen is contained in 
a preliminary description in the ‘Gardener’s Chronicle' f, I cannot pursue the com- 
parison further. Plowright’s fungus must, however, be entirely different, since it is 
associated with Æcidium Periclymeni, Schum., and I have never seen an secidium on any 
genus of the Caprifoliacee in this region. 
The dimensions of the spores of the Indian variety are contrasted with those of the 
European in the following table :— 
AEcidiospores. Uredospores. Teleutospores. 
е ШИСИ ee тыя 26-17 x 21-13 р. 28-19 x 21-16 и; 60-35 x 21-12 p. 
3—4 pores. 
Brachypodium ........ \ 24-20 x 21-15 u; 44-38 x 11-10 р. 
Indian..] _ 31-19 x 24-18 p. Uv bons 
Я) ка гл, 28-22 x 22-20 п; 55-43 x 14-8 и. 
4—6 рогез. 
Тһе zecidiospores of the Indian variety are larger than those of the European; the 
uredospores are much the same in size, but are not associated with paraphyses; and the 
teleutospores are decidedly smaller. | 
The European ecidium is known to occur on several species of Rhamnus, whilst in 
India, in the Simla region, it is known only on one of the four species prevalent. Тһе 
uredo- and teleutospore stages in Europe are known to occur on several genera of 
Grasses, including Festuca; in India they are, so far, known only on three genera, 
though possibly future research may prove their occurrence on more. 
Inoculation Егрегітепів.-Тһе teleutosporie stage on Brachypodium is apparently 
much commoner, and in nature is evidently much more clearly connected with æcidial 
production оп Rhamnus than the teleutosporie stage on the other two hosts; for where- 
ever I found the ecidium on Rhamnus І have usually found Brachypodium with 
teleutospores, though I have frequently, and indeed usually, missed Piptatherum and 
Festuca. The latter are, however, more difficult plants to recognize with certainty 
when only in leaf than Brachypodium. 
(Ехрет. L) I therefore commenced inoculation experiments with teleutospores from 
Brachypodium, and had my first positive result on the 4th July, 1889, on the leaves of 
a twig which I had cut off and placed in water, and on which I had placed sporidia on 
the 25th June. After laying on the sporidia I placed the twig in a glass eardener's-box 
outside, within which the air is always very moist. On the 4th July several leaves 
showed very numerous foci of attack with spermogonia. On the 24th July these leaves 
exhibited young eecidia. ; 
I hadjno further opportunity of continuing these experiments that year, as the teleuto- 
spores refused to germinate any longer, but I resumed them this year (1890). 
25. 
ж Bericht der Deutschen botanischen Gesellschaft, 1888, Bd. vi. Heft 3, p. 1 
t * Gardener's Chronicle,’ July 12th, 1890, p. 42. 
