232 MR. A. ВАВСГАУ ON THE LIFE-HISTORY 
(Exper. IL) I placed some teleutospores from some specimens of Brachypodium 
collected on the 16th January in water on the 10th April: on the 11th I noticed that 
free germination had taken place, with the production of numerous sporidia. I placed 
these on the leaves of а twig of Rhamnus under a glass shade in my laboratory. On the 
16th (5 days) I noticed several foci of attack, though as yet there was no discoloration 
of the leaves, but only characteristeric upheaved spots. On the 19th (8 days) I counted 
40 leaves on the twig inoculated, 21 of which were very distinctly attacked, as many as 
12 distinct foci being observable on one leaf. On another twig, similarly treated, I 
counted 12 leaves, of which 6 were attacked. On the 30th both twigs began to wither 
without making any further progress, and the experiment came to an end. 
(Exper. III.). Again, on the 7th May I inoculated a twig with sporidia derived from 
teleutospores collected on the 26th January, and put in water the preceding day (6th). 
On the 19th May (12 days) I counted 8 spermogonial spots on one leaf, and several spots 
on others; but unfortunately the twig began to wither and the experiment was closed. 
There were immense numbers of foci of attack on these leaves. 
(Exper. IV.) On the 12th April, as a parallel experiment to that described in the pre- 
ceding paragraph, I inoculated several leaves of a twig with sporidia of teleutospores from 
Piptatherum which had been 24 hours in water, and the twig was then put under a 
separate glass shade in my laboratory. On the 17th (5 days) several leaves showed 
decided spots of attack, which on the following day were yellow, bearing spermogonia. 
On the 19th (7 days) I counted 41 leaves on one twig, 7 of which were very distinctly 
attacked, the highest number of distinct foci on any single leaf being 5. Another twig 
had 10 leaves, 2 of which were attacked; and a third had 12 leaves, 3 of which were 
attacked. These twigs also withered before producing eecidia. 
(Exper. V.) On the 23rd May I inoculated two separate twigs with the sporidia of 
Piptatherum or Festuca gathered on the 5th February, and put each under a separate glass 
shade. I purposely abstained from using any spores from Brachypodium for some days 
previously in order to avoid accidental contamination. One of these twigs (A) showed 
one spot of initial attack (distinct spot of upheaval) on the 30th, and on the following 
day two such spots: on the 14th these two spots were distinctly spermogonial ; but there 
were also two other very distinct yellow spermogonial spots on each of two leaves (2. 6. 
four spots in all), and one younger point of attack оп а third leaf. Soon after this the 
twig began to wither, and the experiment was closed on the 21st. Тһе other twig 
(B) showed three spermogonial spots on one leaf on the 6th of June; but the twig was 
attacked by aphides and became sickly in appearance, and the experiment was closed on 
the 14th. 
(Exper. VI.) On the 16th June I inoculated a twig with the dry spores from Pipta- 
therum or Festuca collected on the 5th February and kept it under a glass shade. One 
spermogonial patch was ultimately produced. 
ое tise ge ene : зы І inoculated some leaves with the sporidia of spores 
stad. Rs doses wed e im January, and on the 12th noticed four spots of 
йн йылы ы Somat stage, all on one leaf. The teleutospores were now found to 
855181 In germination, the season for natural attack having come nearly to a close. 
