OF PUCCINIA JASMINI-CHRYSOPOGONIS, 239 
The spores are, like the zecidiospores, very brilliant in colour (orange-red). They do not 
germinate readily in water, but some always do, throwing out a single, long, twisted 
unbranched tube. They are also very large, measuring when fresh 88-28 х 26-23 и, or 
оп an average 2877 x 2675 p. When seraped off they often retain a portion of the stalk 
(РІ. LVI. fig. 21). They are sparsely spiny and contain an indefinite number of germ- 
pores. There are no paraphyses. | 
The teleutospore pustules are well raised, deeply convex, plump, oval or broadly linear, 
and dark brown. ‘They are both epi- and hypophyllous, but oftener epiphyllous. The 
spores are oval, and well rounded at both ends, slightly constricted at the septum, and 
very thick-walled (fig. 15). There is little or no special thickening at the free end. 
They are firmly adherent, and when scraped off retain a portion of stalk. They are dark 
brown, smooth on the surface, and measure 48 x 24 at the septum. А clear nuclear 
vesicle is visible in each cell. Young teleutospores are often seen in fully developed 
uredo pustules. They germinate only after a winter rest. After lying 24 hours in water 
it will be seen that they have thrown out promycelia—one from a point near the apex of 
the spore, and the other from a point near the septum (РІ. LVI. fig. 15), into the distal 
ends of which the orange-red contents of the spore wander, leaving the spore-walls still 
deep brown. Тһе end then divides into four compartments, each producing a sporidium 
at the end of a short pointed sterigma (Pl. LVI. бо. 16). But frequently and very charae- 
teristically, after the promycelium has divided into four parts, the sterigmata form 
uncommonly long tubes, usually much twisted (РІ. LVI. fig. 18, а, 0). In such cases they 
do not form sporidia. I do not know whether a similar growth occurs in nature, but if 
it does the sterigmatous tube must attack directly without the intervention of a spori- 
dium. This curiously abnormal formation of sterigmata is by no means uncommon, and, 
indeed, in water-cultivations it is as common as the more normal course sketched above. 
The sporidium is large, oval, and coloured, and frequently emits a germ-tube before it is 
detached from the sterigma. The germ-tube is usually characteristically spiral, and 
rarely straight (РІ. LVI. fig. 16). Very frequently, instead of a regular germ-tube being 
emitted, the sporidium immediately forms a secondary sporidium (РІ. LVI. fig.17). The 
primary sporidium measures 14 x 12 п, and the secondary about two-thirds this. 
I now proceed to describe some of the experiments I made to establish the connection 
between these two phases of the life-history of the parasite. 
LA. A twig was inoculated on the 7th May with sporidia of teleutospores from 
Chrysopogon, freshly collected and had lain 24 hours in water. On the 20th (13 days) 
small points of attack were noticed, and on one leaf three groups of spermogonia on 
three separate areas of invasion. : pos 
I. в. Another twig of the same branch was inoculated the same day with the sporidia 
of Puccinia Caricis-filicine ; but this twig remained perfectly free from attack. 
Тһе whole branch began to wither on the 27th without twig 1. А making any further 
progress, and the experiment came to an end. 
