210 ‘MR. A. BARCLAY ON THE LIFE-HISTORY 
II. On the 8th May some leaves were inoculated with the sporidia of Puccinia coro- 
nata (from Piptatherum holciforme, Roem. & Schult.). On the 20th (12 days) I noticed 
a single spermogonial patch on one leaf. But as on that date I had obtained clear proof 
from Experiment I. a that Puccinia Chrysopogonis was the related teleutospore, I 
attributed the single spot of attack to the accidental contamination of my inoculating 
material, and re-inoculated the leaves with the sporidia of P. Chrysopogonis, again 
freshly gathered and placed in water 24 hours previously. On the 80th (10 days) several 
distinct spots of attack were observed, and 8 leaves (other than that accidentally inocu- 
lated) presented small orange-yellow spermogonial spots, some leaves with 2 to 4 such 
distinet spots. Оп the following day (31st) I carefully counted the leaves of the twig. 
It had 66 leaves in all, 13 of which bore spermogonial patches; 9 of these latter bore 
each a single spot of attack, whilst 4 bore from 2 to 4 distinet spots each. On the 6th 
June I counted 20 spots of distinet attack on 15 leaves, some very large and typically 
brilliant in colour. On the 26th June most patches bore open ripe æcidia. 
ПІ. On the 23rd May I inoculated a twig with sporidia derived from teleutospores 
gathered on the 11th February and kept in my laboratory since. "The spores had lain 
24 hours in water, and had produced an immense number of sporidia. On the 30th 
(7 days) I noticed several spots of initial attack, i. e. characteristic points of upheaval, 
and one spermogonial patch. Оп the 1st June this twig, which bore in all 90 leaves, 
showed 13 leaves with spermogonial patches. On the 6th June I counted 15 spermo- 
gonial spots on 9 leaves (the other 4 had dropped). On the 19th some well-developed 
young @сїйїа were noticed, but the twig thereafter withered, and the experiment was 
closed on the 21st. : 
IV. On the same date, viz. 23rd May, another twig was inoculated with the same 
material, and kept under а separate shade. Four leaves were found very distinctly 
attacked on the Ist June (9 days) ; on the 6th I counted 86 spots on 17 leaves; on the 
19th many leaves bore young, well-developed ecidia. | 
V. On the 23rd June I inoculated another twig with sporidia derived from the spores 
of Р. Chrysopogonis, freshly gathered, which had lain 24 hours in water. On the 27th, 
when I left Simla for three days, there was no sign whatever of attack; but on my return 
on the 30th (7 days) I found the leaves extensively attacked in the spermogonial stage ; 
18 leaves were so attacked, with brilliant yellow spots, and on one leaf I counted 12 
distinct foci of attack. On the 7th July I counted the leaves again carefully. One 
branch of the twig had 32 leaves, of which 16 were conspicuously attacked ; the other 
branch had 88 leaves, of which 20 were attacked. Thus 36 of 70 leaves were attacked, 
and many in several places. Most of the leaves which escaped attack were the lower 
-older ones. | 
VI. I made only one experiment іп the reverse direction, namely the production of 
uredospore pustules with æcidiospores ; but this was so successful that, taken with the 
evidenee afforded by the preceding experiments, no others were at all necessary. Т 
uprooted a healthy plant of Chrysopogon which I found, and put it into a pot in a 
gardener's box in my verandah. It remained there perfectly healthy for about three 
