Pammel — Anatomical Characters of Seeds of Leguminosae. 145 



forms a continuous even layer, darker in color than the 

 cuticularized layer. In cross-section several canals may be 

 seen extending into the cell-walls from the cuticle. A narrow 

 light line runs close under the cuticularized layer. The cell- 

 cavity gradually tapers upward and is filled with protein 

 grains, tannin, occasionally a large chromatophore, and pig- 

 ment. The cell-walls color blue with chlor-iodide of zinc. 



Osieosclerid. Layer is well developed; cells uniform in 

 width above and below, as a rule with but a slight constric- 

 tion in the middle, 12.6 // to 14 // in length and 11.2 /i in 

 width. Cell-walls greatly thickened and provided with longi- 

 tudinal canals. In sectional view these plainly indicate that 

 they are minute canals, and not folds as has been stated by 

 some writers. 



Nutrient. The layer is unequally developed, from a few 

 rows of cells to a dozen ; the cells are thin- walled, somewhat 

 elongated, compressed, walls colored greenish or purplish. 



Endosperm. The cells of the aleurone layer are longer 

 than broad, thick-walled, containing fat and protein grains. 

 In the underlying cells the primary cell-wall is thin, the re- 

 mainder of the cell-wall constitutes the reserve cellulose and 

 is greatly thickened. Following this is a layer consisting of 

 one or two rows of elongated, thick-walled cells. 



Embryo. The first cells of the first row are smaller; cell- 

 walls which join the endosperm are thickened ; the remaining 

 cells are larger except the procambial vessels. All of the cells 

 contain an abundance of protein, small starch grains, and fat. 

 Cell-walls color blue with chlor-iodide of zinc. — (St. Louis, L. 

 H. Pammel. ) 



Trifolium reflexum, L. 



Fl. IX. f. 5. 



Testa and endosperm 100-110 fx in thickness. The Mal- 

 pighian cells nearly as long as endosperm and remainder of 

 testa are thick. 



Maljpighian. Quite uniformly developed, 42-44 [x in 

 length. Cuticle thickened irregularly ; cuticle and cuticular- 

 ized part separating readily from the remainder of the cells; 

 the light line rather wide under the cuticularized layer; 

 several canals projecting into the cell-wall; cells containing 



