Pammel — Anatomical Characters of iSeecls of Leguminosae. 149' 



in the upper part of the cell. Several pores in each cell 

 project into the wall beyond the light line. Normally the 

 cells as a whole are colored yellow, and contain tannin and 

 some yellow pigment. 



Osteosclerid. Cells usually have a broad base and an inter- 

 cellular space in the upper part of the layer. Cells in sec- 

 tions from the lateral part of seed are more or less I- shaped 

 with the base somewhat broader, longitudinally striated. 

 Cell-walls are colorless and with chlor-iodide of zinc color 

 blue. Some tannin present in the cell-cavity. 



Nutrient. This layer is differentiated into two parts, and 

 is much compressed. Cells elongated, thin-walled ; Cells in 

 the lower part are smaller and contain much more pigment 

 and tannin than those of upper portion. Walls color blue 

 with chlor-iodide of zinc. Vascular elements occur in 

 this layer; these give the lignin reaction. Parenchyma cells 

 surrounding the root cap also give reaction for lignin. 



Endosperm. Aleurone cells very distinct; containing fat 

 and protein. The thick walls consist of reserve cellulose; 

 walls color but faintly with sulphuric acid and iodine ; the 

 non-mucilaginous cells below are elongated, thick-walled, with 

 a small cavity in which some protein remnants occur. In 

 water the waUs become mucilaginous, and change in outline. 

 Internal layer of endosperm of several rows of thick- walled, 

 elongated cells. 



Embryo. The cells of the first row are smaller, with thick 

 exterior walls which color blue with chlor-iodide of zinc. The 

 cells below are larger and somewhat irregular; the portion of 

 the cotyledon next to the endosperm becomes the under side 

 when the seed germinates; the cells of the interior rows 

 where the cotyledons meet, except the outer, are elongated, 

 palisade-like, and become true palisade cells in germination, 

 composing two or more rows which are closely connected 

 with the procambial vessels. Cells of the cotyledons densely 

 filled with protein and fat. 



Iodine alone does not show starch grains because obscured 

 by fat and protein. When treated with chlor-iodide of zinc 

 small round or elliptical starch grains show. — (S. W. Texas, 

 Heller, Mo. Bot. Gard.) 



