Pammel — Anatomical Characters of Seeds of Leguminosae. 161 



found in the lower part of the nutrient layer. The aleurone 

 cells are elongated, and the cell-walls of the endosperm do not 

 color blue rapidly with chlor-iodide of zinc. Oxalate of lime 

 crystals are abundant in the embryo, but the reservoirs for 

 secretion are not so numerous as in the last species. These 

 lysigenetic reservoirs also occur in the cotyledons of the Natal 

 Tephrosia elongata (Coll. by Wood, Mo. Bot. Gard.), the 

 Hawaiian Islands T. piscatoria (Coll. by A. A. Heller), Teph- 

 rosia paUeiis (Bernhardi collection. Mo. Bot. Garden), Teph- 

 rosia spicata (Florida, Curtiss, No. 4823, Mo. Bot. Gard.), 

 lephrosia Schiedeana, Schl. (Fl. Mexico, San Bias, W. G. 

 Wright, No. 1337), and Tephrosia leucantha, HBK. (Prin- 

 gle, PI. Mexicanae). In T. leucantha and T. Schiedeana 

 these reservoirs also occur in the nutrient layer. Crystals 

 were found in all the species except the last two. 



Indigofera, L. 



This genus has been studied by Nadelmann, who did some 

 work on /. iincioj'ia, L. and /. hirsuta, L. The waUs of the 

 endosperm, in their early stages, are thin, and the cells are 

 rich in plastic material. The young cells have what Nadel- 

 mann calls " Schleim-blaschen " imbedded in the protoplasm. 

 The smaller mucilaginous masses unite to form a larger one. 

 From these masses the waU is built up. The primary mem- 

 brane in young seeds colors deep blue with sulphuric acid and 

 iodine. The secondary membrane colors light blue. The 

 tertiary membrane forms much later. It is noteworthy that 

 the mucilaginous endosperm at no time showed transition 

 starch. The endosperm exceeds the testa by five times its 

 width in /. tinctoria and /. hirsuta. The white color of the 

 seed, in cross-section, is due to the mucilaginous endosperm. 

 The young flowers of /. tinctoria at the time of flowering 

 contain indigo blue, known as indigotin. 



Indigofera leptosepala, Nutt. 



PI. XIII. f. 1-1 c. 



Testa and endosperm vary in thickness from 83-866 fi. 

 The cotyledons are 680 ^u, across, and uniform throughout. 

 Malpighian. CeUs larger than the remainder of the testa, 



