Pammel — Anatomical Characters of Seeds of Leguminosae. 193 



lowed by ordinary parenchyma. Procambial vessels not lig- 

 nified. Cells contain an abundance of fat, protein, and large, 

 stratified starch grains. 



Phaseolus multiflorus, Willd. 



PI. XXI. f. 2-2g. 



A full account of this seed is given by Mattiroio and Bus- 

 calioui. Tschirch and Oesterle, Harz, Holfert, and Haber- 

 landt also treat the anatomy of the seed briefly. A general 

 account is also given by Lubbock, and by Sachs, who studied 

 its germination. 



Holfert distinguishes five layers of the testa: (1) Mal- 

 pighian, (2) osteosclerid, (3) obliterated nutrient layer, (4) 

 star-shaped parenchyma, (5) obliterated star-shaped paren- 

 chyma (verfilztj. The testa is spotted, much larger than 

 in P. perennis, laterally 580 fi thick, towards the edges 770 /x 

 thick. 



Malpighian. The cells are 84 /a long. Cuticle smooth; 

 cuticularized layer not strongly developed ; light line runs 

 close under the latter ; pores project into the wall below the 

 light line. Cell-cavity large at the base, narrowed rather 

 abruptly above. Walls of some of the cells as well as the 

 cavity contain a bluish pigment, especially in the lower por- 

 tion. The walls, cuticularized layer, and light line color 

 blue with chlor-iodide of zinc. 



Osteosclerid. Cell- walls thickened, longitudinally striated. 

 Cells I-shaped, with elongated intercellular spaces. Cells 

 contain some pigment. 



Ifutrient. Layer differentiated into two parts ; cells of the 

 upper portion thin-walled, loosely arranged, with numerous 

 intercellular spaces ; but little pigment present. Cells of the 

 lower part narrower, thicker-walled, with more pigment, and 

 tannin. Vascular elements occur in this part of the nutrient 

 layer ; these are also pigmented. The walls of the vessels 

 give the reaction for lio;nin. The walls of the other cells 

 of the nutrient layer color blue with chlor-iodide of zinc. 



Mycotic. In cross-section the cells are closely packed 

 and would pass for ordinary parenchyma ; a tangential sec- 

 tion, however, shows that the cells are elongated, somewhat 



