ON TUBIFEX. 155 



Fig. 3. — Invagination of cell to form seta (diagrammatic). 

 4 & 5. — Further stages of ditto. 



6. — Gregarina s?enuridis encysted, showing partition. 

 7. — Ditto, ditto, enclosing vesicles. 

 8. — Pseudo-navicula. 



9. — Gregarina saenuridis in act of conjugation (Geg.) ; a and 6, 

 the two individuals ; c.c, their nuclei. 



10. — Young worm within vitelline membrane. 



11. — Pole of capsule with end broken off by exit of young worms. 



12. — Capsule, with young worms. 

 13. — Young worm, immediately after its escape from the capsule. 



IResults ot a /llMcroscoptcal Jnvesttoation of tbe 

 Hction ot Bmmonium /iDoli^bbate ant) otber 

 Cbemtcal Hgents on tbe Dascitlar ant) Cellular 

 Tlissues ot about 120 Bitferent plants. 



By Thomas Spearman Ralph, M.D., Pres. Mic. Soc, Vict. 



THE investigations I have made with chemicals on Vegetal 

 Tissues, and which form the subject of my present 

 communication, have arisen out of an examination of 

 various plants with reference to their protoplasmic cell-con- 

 tents. Simultaneously with this, I was carrying on an enquiry 

 after further evidence of the presence of Bacilli in the tissues of 

 living plants, a subject to which I directed attention last year in 

 May. These investigations have been in great measure closely 

 related, but the leading feature has been the investigation of the 

 action of chemicals on the cell-contents of leaves. With regard 

 to Bacilli, or Bacterial forms, besides their occurrence in Vallis- 

 neria cells (and concurrent with the phenomenon of cyclosis), I 

 have met with these organisms in A?iachartSy and in the cells of 

 Arimi CEthiopium (Richardia ?), Vinca major (variegated form), 

 Erythriiia^ and in the leaves of Tea after infusion. 



The experiments on protoplasm recorded by Mr. Gardner in 

 the Qicart. Journal of Microscopical Science^ induced me to make 

 further experiments, and briefly, I have to state, I have employed 



