38 THE MICROSCOPE 



2.--Picro-Carmine and Safranine.— This is especially applic- 

 able where you wish your specimen, owing to its structure, etc., 

 to be very clear and transparent. Stain in picro-carmine first, 

 then with saft-anine. The former stains all the connective tissue 

 and nuclei, while the latter stains muscle, epithelium, etc. 



3.— Picro-Carmine and Iodine Green.— This is one of the 

 most useful combinations of which we know. Stain the sections 

 in picro-carmine, wash well in water slightly acidulated with acetic 

 acid, then stain in a watery solution of iodine green, take care 

 they do not become overstained, which can easily be ascertained 

 by washing them in water. If a section, say of the posterior 

 third of the Tongue, be stained, all the connective tissue and the 

 muscles will be red, whilst the mucous glands and adenoid tissue 

 will be green. The serous glands do 7Wi take up the green stain, 

 therefore the combination is of utmost value for gland tissue. 

 Most exquisite effects are produced in the cerebellum, bone, and 

 intestine by this method. The sections are generally mounted in 

 dammar, or Canada balsam and benzole. Sections stained in log- 

 wood and iodine green, and mounted in dammar, are very good. 

 The acini of the mucous glands are stained a bright green, while 

 the epithelium in the different ducts is of a logwood tint. A high 

 power shows the nuclei stained with logwood. 



4.— Eosin and Aniline Blue give good results, but require to 

 be used cautiously, as, if the staining is too deep, the section 

 becomes opaque. The section should be very thin, and must be 

 well washed after staining with eosin, and then just immersed for 

 a few seconds in aniline blue. 



6. — Eosin and Logwood are very good for staining the zone of 

 ossification in growing bone. The sections of decalcified bone are 

 first immersed for a few days in a J % solution of chromic acid, or 

 I % solution of bichromate of potassium, soak the sections in i % 

 solution of carbonate of soda for lo — 20 minutes, which will be 

 sufficient ; wash well. Prepare two watch-glasses, containing a 

 dilute solution of logwood, place the sections in one, let them 

 remain for a minute, stir them round, and then place them in the 

 other ; stain till deep enough. This prevents a deposit of granules 

 over all the sections ; then, after washing well with water, stain in- 



