110 THE MICROSCOPE 



Corrosive Sublimate for Brain, etc— After the preparation has 

 been hardened in Miiller's fluid, instead of putting it in alcohol, 

 place it for some days in a 5 per cent, solution of corrosive sublimate, 

 which is renewed every day until the solution is no longer coloured. 

 If left too lo?ig the preparation becomes black, or if ^wt long enoiigh 

 small black points appear. It is very elastic and firm, and very 

 thin sections can be cut ; it stains very well without ammonia, 

 carmine, etc. 



Saure-gelb, Chrysoidin, Rocellin, etc.*— The first colours bone 

 a beautiful orange, tracheal cartilage, and connective tissue, lemon 

 colour, is not suitable for chromic acid preparations. Chrysoidin 

 is useful for bone and all kinds of connective tissue, which it colours 

 a bright yellow. Its best effect is on fresh preparations. Bismark 

 Brown has its best effect with nuclei (either alcoholic or chromic 

 acid preparations), and unicellular organisms, bacteria of all kinds, 

 colourless blood corpuscles, etc. 



Rocellin colours bone, muscle, connective tissue, glands, and 

 epithelium cherry-red ; gold or orange serves for fresh or alcoholic 

 or chromic acid preparations. Bone is stained deep orange red, 

 cartilage, gold, connective tissue, reddish ; especially valuable for 

 glandular tissue ; it gives a splendid appearance to liver injected 

 with Berlin blue, the blue vessels showing on a gold ground ; 

 sections of skin give fine results. Preparations after washing 

 and cleaning are best mounted in Canada Balsam ; oil of cloves is 

 mostly used for cleaning, but where the colours are very delicate, 

 use oil of Lavender or quite colourless oil of Aniseed, as the 

 yellow colour of the oil of cloves injures them. 



Staining with Rose Bengale, Iodine Green and Bleu de Lyon. 



— If dissolved in water it is very useful for staining Chromic Acid 

 preparations — e.g. — Spinal Cord, the grey substance of which is 

 stained a deep red, while the white substance is paler. It is also 

 adapted for muscles and connective tissue of Vertebrata and 

 Invertebrata, but not satisfactory for glandular tissue or bones. It 

 is especially suited for double and treble stainings, in conjunction 

 with Iodine green, and Iodine green and an aqueous solution of 



* Mikr. Anat, xxii. (1883), p.p. 132-142. 



