AND HOW TO USE IT. 183 



per cent, solutions are excellent re-agents. An exposure of from a 

 quarter to half an hour or more is an extremely useful means of 

 isolating muscular and nerve elements, glandular passages, and 

 even ordinary ciliated and olfactory cells. 



Chloride of Iron is used by Billroth and Fiihrer for hardening 

 spleen, which becomes sufficiently hardened in from one to two 

 hours in a solution of the colour of Madeira or Malaga wine. 



Chloride of Mercury.— The chemical effects of the sublimate 

 are well known. Macerating for several days in a solution of this 

 salt may be advantageously used for hardening and isolating the 

 axis cylinders. This re-agent forms an element of several very 

 serviceable preservative fluids. 



Chloride of Platinum hardens and gives flattened organs a 

 diffused yellow tinge. Equal portions of chromic acid and chlo- 

 ride of platinum (each i '400) are recommended for the connective 

 tissue framework of the retina. 



Alcohol and Acetic Acid renders spinal cord marvellously 

 clear, even in a few hours, and permits many things to be better 

 recognised than by any other methods. The recipe is, naturally, 

 to be modified according to necessity. The proportions usually used 

 are 3 parts alcohol with i part acetic acid (L. Clarke). 



Moleschott's Strong Mixture modified from Clarke's Method. 



Strong acetic acid (I'oyo s.g.), i part; alcohol (o*8i5 s.g.), i part; 

 distilled water, 2 parts. Very serviceable for hardening many 

 organs ; causes connective tissue portions to become transparent, 

 renders albuminous matters distinctly prominent. 



Beale's Alcohol, Acetic, and Nitric Acid, for Examination of 

 Epithelial Structures, etc. — Water, i ounce ; glycerine, i ounce ; 

 spirit, 2 ounces ; acetic acid, 2 drachms ; hydrochloric acid, h 

 drachm. 



Alcohol and Soda. — Eight to ten drops of caustic soda to each 

 ounce of alcohol. Tissues are rendered very hard and transparent, 

 particularly adapted for investigating calcareous matter deposited 

 in various morbid processes ; also, in tracing the stages of ossifica- 



