234 THE MICROSCOPE 



of rose-aniline hydro-chloride 2 grains ; methyl-blue, i grain ; rub 

 up in a mortar. Then dissolve aniline oil, 3 cc, in rectified spirit. 

 Add the spirit slowly to the stains until all is dissolved, then 

 slowly add distilled water, 15 cc. ; keep in a stoppered bottle. 

 (For method of using, see Tubercle Bacillus.) 



Ferrier's Magenta Fluid.— Magenta crystals^ i decigr. ; dis- 

 tilled water, 15 cc. Dissolve and add rectified spirit, 5 cc. ; 

 glycerine, 20 cc. Useful for blood-corpuscles. Being of a speci- 

 fic gravity, similar to that of the Liquor Sanguinis, the coloured 

 corpuscles of non-mammalian vertebrates alter but little in shape 

 while they become stained. 



Magenta. — Gibbes' Formula is a reliable stain ; it does not 

 fade like the others. Prepare as follows : — Magenta crystals, 2 

 parts; pure aniline, 3 parts; alcohol (sp. gr., '830), 20 parts; 

 aqua dist, 20 parts. Dissolve the anifine inspirit; triturate the 

 magenta in a glass mortar to a fine powder, mix the spirit gra- 

 dually while stirring, until all the colour is dissolved, then mix the 

 water slowly, still stirring, and put in a stoppered bottle. 



Ditto. — Ransome's Formula : — Rose aniline^ 3 parts ; alcohol 

 anhyd., 42 parts ; magenta or fuchsin, i part ; distilled water, 

 45 parts. Mix and preserve as above. 



Gibbes' s Rose-aniline Acetate.— Must be rubbed up in a 

 mortar with rectified spirit, and when thoroughly dissolved an 

 equal quantity of distilled water added ; 5 grams of crystals to 

 100 cc. of spirit, and thoroughly dissolved ; then 100 cc. of dis- 

 tilled water added. This makes a good strength for general use, 

 and is a useful stain for blood. 



Aniline Blue (insoluble in water). — By treating it with sulphu- 

 ric acid, the soluble blue may be obtained. This may be simply 

 dissolved in water until it assumes a deep cobalt colour, or the 

 following solution may be prepared : — Soluble aniline blue, 2 

 centgr. ; distilled water, 25 cc. ; alcohol, 20 to 25 drops. This 

 fluid stains tissues preserved in alcohol a brilliant blue in a few 

 minutes, but those preserved in chromic acid are coloured less 

 rapidly. Tissues stained with this colour may be preserved in 

 water, alcohol, and glycerine, and are not altered by the addition 



