in, 2. Mi not: Notes on Histological Technique. 173 



Der Api);irat kann nach Herausnahme des Heisshiftrohres und Er- 

 satz desselben durch ein kürzeres ohne Klappe, welches gleichzeitig 

 einen Support für die Aufstellung eines BuNSEN'schen Gasbrenners trägt, 

 sofort auch für Gasregulatoren verwendet werden. 



In der hier beschriebenen Form, auch mit eventuellen Modifica- 

 tionen und der facultativen Einrichtung für Gasregulation, wird der 

 ganze Apparat sammt Lampe, Regulator und Thermometer geliefert von 

 Herrn Spenglermeister Hager, Bern, Lorraine Centralweg. 



Notes ou Histolog'ical Teclinique. 



By 



Charles S. Miiiot, 



Boston, Mass. 



When the new histological Laboratory of the Harvard Medical 

 School in Boston was initiated, it became necessary to bring into use 

 not only the advaneed methods which are indispensable in investigations 

 but also those methods best suited for the iise of ordinary medical 

 students in class - work. As it is by the latter that the laboratory is 

 most used, attention has been directed towards improving the con- 

 venience of methods. The following notes describe such of the im- 

 provements thus made as seem worth Publishing, together with accouuts 

 of a new Solution of carmiue, and a new method of mounting series 

 of celloidine sections , which is far less elabourate than that recently 

 described by Weigert*. 



A part of what is here given has already appeared in the Amer- 

 ican Naturalist, (August, and September, 1885) and in Dr. Whitman's 

 valuable „Methods". 



1. AI CO hol. My experience has lead me to question whether ab- 

 solute alcohol, which retails in America at one doUar a quart, is I will 

 not say a necessity, but even an advantage for the preservation or 

 hardening of histological material, or at least of vertebrate tissues. 

 One frequently encounters the direction to employ absolute alcohol in 

 conjunction with this or that method. After repeated tests I have 

 found 96 Yo entirely sufficient for all the manipulations for which the 



') Weigert, This Journal, Vol. II, 1885, p. 490. 



