Eumycetes. 227 



The swarmspores of S. Piierariae, liberated on the surface of 

 the host, are attracted by the chemical substances diffused out from 

 its stomata or water-pores, enter through these into the subepidermal 

 tissue, infect the cells containing the stimulating substance and then 

 grow out to a spherical orange yellow, semifluid body. Each swarm- 

 spore contains a nucleus with an indistinct membrane and some 

 granules, of which the largest one represents the chromatin-nucleo- 

 lus. During the vegetative period of growth, many secondary nu- 

 cleoli are formed within this primary nucleolus and go out into the 

 nuclear cavity. The secondary nucleoli contain at first chromatin 

 uniformljs as proved b}^ colour-reaction, but afterwards it goes to 

 their inner periphery, becomes detached from the nucleoli and scat- 

 tered in the nuclear cavity as chromatin-granules, this process is 

 also very clearly seen in the primary nucleolus. Hence the writer 

 comes to the conclusion that here both primary and secondary nu- 

 cleoli are the organs for the manufacture of chromatic substance by 

 condensation. Successive nuclear divisions then occur in the fungus- 

 body, whereb}^ it passes to the multinucleate reproductive period of 

 its existence. All these divisions are mitotic and agree in their details, 

 At first, the nucleoli, both primär}^ and secondary, continue to 

 discharge chromatin-granules into the nuclear cavity, the primary 

 one produces pseudopodia-like processes, five chromosomes are for- 

 med from these chromatin-granules (odd number of chromosomes in 

 vegetative cells!), the spindle appears (intranuclear!), and the nuclear 

 membrane disappears, After the Splitting of each chromosome and 

 the migration of daughter-chromosomes to two poles, the latter 

 agglomerate to an irregulär mass at each pole. The stretched spindle, 

 which is constricted at its middle part is broken down there and 

 then the writer has observed the remarkable fact, that each half of 

 the broken spindle attaches to and is gradually absorbed into each 

 daughter chromosome-mass. The next important fact observed is the 

 formation of the nuclear membrane. At one end of the spherical 

 hyaline space around each daughter chromosome a dense c3'toplas- 

 mic mass appears, which then changes into a prominent aster with 

 one, two, or more centrosome-like granules at its focal region. The 

 hyaline space becomes pyriform and comes by its pointed end in 

 close contact with the aster, whereupon the formation of the nuclear 

 membrane around the hyaline space takes place, the process begin- 

 ning with the pointed end and gradually proceeding towards the 

 other. After the complete delimitation of the newly-formed nucleus, 

 the aster and the centrosome-like granules disappear. The writer 

 comes to the conclusion, that the above described centrosphere-like 

 body is a transitory structure concerned in the formation of the 

 nuclear membrane and proposes for it the name of "karyodermatoplast". 



After some nuclear divisions, the cytoplasm of the fungus-body 

 undergoes successive cleavage or is divided simultaneousl}^ into many 

 polyedral masses; in each of these multinucleate partition-products 

 (primordial sporangia) 5 — 6 successive nuclear divisions follow and 

 then the cytoplasm of each sporangium thus produced gives rise 

 to 200 — 300 swarm spores, 



The cytology of S. decipiens agrees perfectly well with what the 

 writer has observed in 5. Puerariae and differs in many respects 

 from the Statement by Stevens. 



In the PostScript the writer discusses the new publications of 

 Stevens and Griggs on S. decipiens on the ground of his investi- 

 gations. S. Ikeno. 



