398 W. Wen die r: Chemische Physiologie 1921— 1922 [38 



trichum Gossypii, and Penicillium italicum. The aetivity of the eiizyme as 

 produced under these eonditions was measured in NaOH-H3P04 buffer Solu- 

 tions having H-ion concentrations from j^H 3,0 to pH 11,0. An attempt has 

 loeen made to determine the effect of the reactions of the culture Solution upon 

 the accumulation of amylase by the various organisms. — From the results 

 the following eonclusions can be drawn: 1. A relation, whicb varies with the 

 organisms seemed to exist between the H-ion concentration of the medium 

 ^nd the accumulation of extra- and intracellular amylase. 2. Fusarium sp., 

 maximum total accumulation was produced in the Solutions having an initial 

 of pH 4,5 and a final reaction of pH 7,8, whereas in Colletotrichum Gossypii a 

 culture Solution with an initial of pH 7,0 and a final reaction of pH 7,9 afforded 

 maximum results, but only slightly less accumulation occurred at pH 8,2. 

 Culture Solutions of pH 3,0 and pH 4,5 were equally favorable in the case of 

 Penicillium italicum. 3. Amylase accumulated more abundantly in the cultures 

 of C. Gossypii than the other fungi studied. 4. A gradual decrease in the amylase 

 accumulation was effected by Fusarium as the culture Solution became more 

 alkaline, this decrease not being coincident with a reduction in the amount of 

 growth. 5. An increase in accumulation occurred in the extra- and intracellular 

 amylase of C. Gossypii as the nutrient Solutions became less acid, neutral or 

 alkaline Solutions being most effective. 6. The intra- and extracellular amylase, 

 produced by any one fungus under varying H-ion concentrations of the culture 

 Solutions, had similar properties with respect to the effect of the reaction of 

 tbe NaOH-H3P04 buffer Solution upon activation. 7. An optimum zone of 

 aetivity, between pH 3,0 and 6,0, existed for P. italicum, while in the other 

 fungi the optimum was more sharply defined at pH 6,0 when the aetivity was 

 measured in the buffer Solution at 28*^ C for 24 liours. 8. Complete inactivation 

 occurred at pH 8.0 for the amylase of P. italicum. Under similar amounts of 

 amylase accumulation by Fusarium and C. Gossypii, inactivation was effected 

 by Solutions of pH 9,0 to 1 1,0. 9. A decrease in the actual acidity of the culture 

 Solution occurred in all of the series of P. italicum and all but the most alkaline, 

 or pH 9,2, series of Fusarium and C. Gossypii. The former produced no change 

 in the reaction of this culture Solution, while the latter caused a slight shift 

 toward neutrality. 



360. Karror, P. Der Aufbau der Stärke und des Glykogens. 

 <Naturwissensehaften i), 1921, p. 399—403.) 



361. Kaufnian, ■\Vaiida. Sur les variations du contenu d'amidon 

 dans les graines de pollen du noisetier (Cory/us üve//ana). (Bull, intern. 

 Acad. Polon. Sei. et Lettres Cracovie, cl. sei. math. et nat., ser. B, 1921, ersch. 

 1922, p. 191—198.) — Ref. in Bot. Ctrbl., N. F. 3, 1924, p. 195. 



362. Kohler. Denise. Variation des acides organiques au cours 

 de la pigmentation anthocyanique. (C. R. Acad. Sei. Paris CLXXII, 

 1921, p. 709—711.) — Ref. in Bot. Ctrbl., N. F. 1, 1922, p. 71—72. 



363. Kostytcliew, S. und AfanassjeAva, M. Die Verarbeitung ver- 

 schiedener organischer Verbindungen durch Schimmelpilze bei 

 Sauerstoffmangel. (Jahrb. f. wiss. Bot. LX, 1921, p. 628— 650.) — Ref. 

 in Bot. Ctrbl., X. F. I, 1922, p. 202. 



364. Kozlowski. Aiitoine. Sur l'origine des oleoleucites chez 

 les hepatiques ä feuilles. (C. R. Acad. Sei. Paris CLXXIII, 1921, p. 497 

 l>is 499.) — Ref. in Bot. Ctrbl., N. F. I, 1922, p. 132. 



