180 PROCEEDINGS OF THE AMERICAN ACADEMY 



tearing the body into fragments. Yet globules lying in contact in the 

 body do not unite by moderate pressure, but regain their form when 

 the pressure is relieved. A strong pressure causes them to run to- 

 gether. These facts are compatible with the absence of a proper 

 enveloping membrane. No structure of any kind is visible in the 

 globules. Their color, when isolated, is generally orange red or yel- 

 low ; they range, however, from brownish red to an entirely colorless 

 condition, in different specimens. As the carapace has frequently a 

 tinge of red or green, the color of the oil is of course affected when 

 seen through it. 



" Finding that the comparatively large size of the crustaceans al- 

 lowed of their almost perfect separation from the other bodies sus- 

 pended in the water, by means of a suitable filter, a quantity was 

 collected from a Cochituate service-pipe, and thoroughly washed with 

 distilled water. They were then introduced, mostly in a living state, 

 into distilled water, in an open vessel. In about half an hour the 

 water began to acquire an odor, and after some hours both the odor 

 and taste resembled closely the peculiar flavor of the Cochituate. In 

 a day or two a decided fishy flavor was developed. The water was 

 now somewhat milky, and on microscopic examination an abundance 

 of colorless oil globules were seen diffused through it, with some 

 gelatinous matter, derived from the bodies of the dead crustaceans, 

 with the fragments of which, together with exuviae, the bottom of 

 the vessel was covered. A large proportion were still living and 

 active. In about a week, the water began to regain its clearness, and 

 the odor and taste nearly disappeared. Many of the crustaceans 

 were still alive, and it was noticed that a progressive diminution in 

 the general amount of oil contained in their bodies was evident in the 

 successive examinations. 



" In the Cyclops, these globules are found equally in both sexes, 

 and cannot therefore be derived from the ova ; in many of the fe- 

 males, the granular ova-masses in the internal ovaries are seen in 

 company with the globules, but they are not in connection, nor is 

 there any indication of a transition from one form to the other. In 

 the Daphnia, the small pellucid globules which constitute the ear- 

 liest stage of the ova, and also the hibernating eggs, are visible in 

 many specimens, and do not resemble the globules under considera- 

 tion. The male Daphnia is rarely seen, and I do not know whether 

 the oil is found in both sexes, as in the Cyclops". 



