Table l.-DMNA, nitrite, and NaCl content of salmon roe 

 samples prepared from different species of salmon at various 

 processing plants in the Pacific Northwest. 



to 100 jul. The chromatograms showed few, if any, 

 indications of other volatile A^-nitrosamines 

 studied. 



The multidetection method of Fazio, Howard, 

 and White (1971) was used to prepare four runs of 

 the same sample. The eluants from four silica gel 

 columns were combined into a 1-liter Kuderna- 

 Danish apparatus and concentrated to 1 ml and an 

 aliquot injected for GLC analysis. The concentrate 

 represented 100 g/ml of roe instead of the usual 25 

 g/ml. The increase in the area and height of the 

 NDMA peak was very pronounced. The extract 

 was submitted to an acid-Celite column cleanup. 

 Interferring peaks were removed, but the sus- 

 pected NDMA was still present (Figure 2). 



In view of the above findings, it can be concluded 

 that less than 5 ppb of apparent NDMA was found 

 in salmon roe products of the different species of 

 salmon having been processed at five major loca- 

 tions, and that no other nitrosamines were 

 evident. 



Acknowledgments 



We express our appreciation to Donald M. Cros- 

 grove of NCA, Northwest Laboratories, for fur- 

 nishing us with the salmon roe samples and the 

 respective residual nitrite and chloride data. 



lOOg roe/ml concentrate 



TIME 



Figure 2.-Chromatograms of an extract of nitrite-treated 

 salmon roe before and after being cleaned up on a column of acid 

 Celite. 



Literature Cited 



Fazio, T., J. N. Damico, J. W. Howard, R. H. White, and J. 0. 

 Watts. 

 1971. Gas chromatographic determination and mass spec- 

 trometric confirmation of A^-nitrosodimethylamine in 

 smoke-processed marine fish. J. Agric. Food Chem. 

 19:250-2.53. 

 Fazio, T., J. W. Howard, and R. H. White. 



1971. Multidetection method for analysis of volatile A^- 

 nitrosamines in foods. Proc. Heidelb. Meet. Nitrosamines, 

 Int. Agency Res. Chem., 13-15 Oct. 

 Howard, J. W., T. Fazio, and J. 0. Watts. 



1970. E.xtraction and gas chromatographic determination of 

 A^-nitrosodimethylamine in smoked fish: Application to 

 smoked nitrite-treated chub. J. Assoc. Off. Anal. Chem. 

 53:269-274. 



D. F. Gadbois 

 E. M. Ravesi 



R. C. LUNDSTROM 



Nortliea.'<t Utilization Reaearch Center 

 National Marine Fisheries Service, NOAA 

 P.O. Box 61 

 Gloucester. MA 019.i0 



686 



