FISHERY BULLETIN: VOL. 74, NO. 1 



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anchovy larvae on Tisbe will be given in a sepa- 

 rate section. 



To obtain grovvi;h rates, 15 or more larvae were 

 removed every other day from each tank, then 

 measured, rinsed in distilled water, dried, and 

 weighed in groups of 15. 



CULTURE 



Not until the fifth experiment was the proce- 

 dure developed sufficiently to rear anchovy 

 through metamorphosis. The first four experi- 

 ments ended when it became obvious that it 

 would be impossible to rear them to metamor- 

 phosis because of slow growth and high mortality. 

 Data are included from the first four experiments 

 to provide the background information for the 

 final successful rearing procedure. 



In all experiments, a single inoculation of 40 

 liters of Gymnodinium splendens (1,500-2,000 

 cells/ml) was given at age days. This was 

 sufficient to provide a final density in the tank in 

 excess of 100 cells/ml for about 12 days. Gym- 

 nodinium was cultured using techniques de- 

 scribed by Thomas et al. (1973). If fed only 

 Gymnodinium, survival of anchovy larvae re- 

 mains high for at least 12 days (about 45% at 12 

 days) but growth is depressed (Hunter in prep.). 



In all experiments Brachionus was added on 

 the 4th or 5th day in numbers calculated to yield 

 a density of 30 to 50/ml in the tank (Table 1). 

 Subsequent additions were made daily or on 

 alternate days until day 20 in all experiments. 



Nannochloris sp. was used to culture the rotifer 

 Brachionus (Theilacker and McMaster 1971), and 

 as a consequence Nannochloris was added to 

 larval rearing tanks in all experiments to main- 

 tain a food supply for the rotifers. Four liters 

 (about 13,000 cells/ml) were added on days 4 and 

 5, and further additions were made on the basis of 

 water color. If water in the rearing tank was 

 faintly green none was added as I wished to avoid 

 creating a bloom in the tank because it is difficult 

 to see the larvae in a dense bloom. To avoid a 

 bloom usually required a reduction in the quan- 

 tity added after 20 days. Nannochloris sp. is too 

 small (about 7 /xm) to be directly fed upon by 

 larval anchovy although larvae might ingest cells 

 accidently. 



In experiments 1 and 2 Artemia was added at 

 about 20 days and the level of Brachionus was 

 allowed to slowly decline thereafter. In experi- 

 ment 3, Brachionus was maintained at a high 



82 



