U.S. Department of Commerce. 



1967. U.S. Coast Pilot 5, Atlantic Coast, 301 p. 

 Waller, R. A. 



1961. Ostracods of the St. Andrew Bay system. M.S 

 Thesis, Florida State Univ., Tallahassee, 46 p. 



nelson may 



Lee Trent 



Paul J. Pristas 



Gulf Coastal Fisheries Center Panama City Laboratory 

 National Marine Fisheries Service, NOAA 

 Panama City, FL 32401 



PHOSPHOGLUCOMUTASE POLYMORPHISM 

 IN TWO PENAEID SHRIMPS, 

 PENAEUS BRASILIENSIS AND 

 PENAEUS AZTECUS SUBTILIS 



In a search for subpopulation differences within 

 species of penaeid shrimp in the northern Gulf of 

 Mexico, Proctor et al. (1974) and Marvin and 

 Caillouet (1976) reported genetically con- 

 trolled polymorphism in the enzyme phospho- 

 glucomutase (PGM) in Penaeus aztecus (brown 

 shrimp) and P. setiferus (white shrimp). The 

 brown shrimp were collected in the northern Gulf 

 of Mexico, so they are P. aztecus aztecus Ives, ac- 

 cording to Perez Farfante (1969). The white 

 shrimp, collected both from the northern Gulf and 



from the North Edisto River, S.C., are P. setiferus 

 (Linnaeus), according to Perez Farfante (1969). 

 Our paper describes similar polymorphisms in 

 PGM in two more penaeids, P. brasiliensis Lat- 

 reille and P. aztecus subtilis Perez Farfante. 



Methods 



Specimens were collected off the coasts of 

 Guyana, Surinam, and French Guiana, South 

 America, on cruise 49 of the Oregon II, between 

 lat. 6°13' and 6°29'N and between long. 53°10' 

 and 53°36'W, at 22-29 fathoms, on 9 and 10 Feb- 

 ruary 1974. They were stored at -20°C or below 

 until analyzed. Preparation of abdominal muscle 

 extracts, electropherograms of general protein 

 patterns, and PGM zymograms followed pro- 

 cedures used by Procter et al. (1974). Each speci- 

 men was identified to species by morphological 

 characteristics, then their distinctive general 

 protein patterns (Figure 1) were used to confirm 

 this identification. To do so, each gel was sliced 

 horizontally into two halves after electrophoresis 

 was complete. One half was treated with PGM 

 specific stain and the other half was stained with 

 Coomassie Blue.^ Specimens of P. aztecus aztecus 



'Reference to trade names does not imply endorsement by 

 the National Marine Fisheries Service, NOAA. 



P. brasiliensis 



P. aztecus aztecu 



P. setiferus 



P. aztecus subtilis 



t 



Figure l. — Electropherc^ram showing general protein pattern of Penaeus brasiliensis, P. aztecus subtilis, P. aztecus aztecus, and 

 P. setiferus. Stain used was Coomassie Blue. Direction (f) of protein migration toward the anode (+) is shown. 



453 



