WEINSTEIN and YERGER: PROTEIN TAXONOMY OF CYNOSCION 



all of the bands observed on the gels were taken as 

 "characters" and their percent occurrence as 

 "character states." The data utilized in computing 

 taxonomic distances are summarized in Table 1, 

 and the results of such an analysis in Table 2. All 

 data were "standardized" to have a mean of and 

 a variance of 1 (indicated by £)• Values of d are 

 interpreted as follows, "The larger the distance, 

 the smaller the degrees of association or correla- 



Table 1. -Percent occurrence of banding patterns derived from 

 whole serum samples of seatrouts (Cynosciou). A dash indicates 

 the absence of that band. 



Serum C. nothus C. arenarius C. regalis C. nebulosus 



band n = 34 n = 19 n = 19 n = 500 



Table 2.-Taxonomic distances (d,^) calculated for interspecific 

 comparisons among four seatrouts (Cynoscion). The larger the 

 value, the smaller the degree of association or correlation 

 between taxa (Sokal 1961). 



Species compared dj 



C. nothus versus C. arenarius 

 C. nothus versus C. regalis 

 C. nothus versus C. nebulosus 

 C. arenarius versus C. regalis 

 C. arenarius versus C. nebulosus 

 C. regalis versus C nebulosus 



63.8 

 44.0 

 78.5 

 54.7 

 67.3 

 72.9 



tion between taxa!' (Sokal 1961). Based on the 

 calculated distances (Table 2), C. nebulosus has 

 diverged to a larger extent than any other 

 member of the genus and differs from the others 

 by about the same order of magnitude. Cynoscion 

 nothus and C. regalis apparently share a closer 

 relationship in blood protein patterns than do C. 

 regalis and C. arenarius, a result comparable to 

 that based on osteological similarity (Mohsin 

 1973). It also seems apparent that the close cor- 

 relation between C. yiothus and C. regalis (44.0) 

 and between C. arenarius and C. regalis (54.7) 

 should imply a similar distance between C. nothus 

 and C. arenarius. Such is not the case; and the 

 value 63.8 apparently indicates that their differ- 

 ences are even greater than their similarities. 



Eye Lens Proteins 



Eye lens preparations exhibited considerable 

 uniformity of pattern (Table 3, Figure 3). Four 

 bands designated by arable numerals were shared 

 in common by the seatrouts; however, the amount 

 of protein in each band differed significantly. For 

 example, C. regalis had a greater protein concen- 

 tration in band 1 than did any of the others. The 

 quantity of protein in this band was not observed 

 to differ significantly in any of the samples 

 processed. Bands 1 and A in C. nebulosus {n = 275) 



Table 3.-Percent occurrence of banding patterns derived from 

 eye lens nuclei of seatrouts {Cynagcion). A dash indicates the 

 absence of that band. 



Band 



C. nothus C. arenarius C. regalis C. nebulosus 

 n = 35 n = 12 n = 16 n ^ 275 



+ A B C D 



Figure 3.-Diagrammatic representation of protein bands 

 occurring in eye lenses of four seatrouts. Arabic numerals 

 indicate bands shared by all taxa (similar electrophoretic 

 mobility). Letters indicate bands that are either unique or not 

 shared by all members of the genus. (A) Cynoscion nothus, (B) C. 

 arenarius, (C) C. regalis, (D) C. nebulosus. 



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