

A 

 AB 



Hybrids C.opilio C.bairdi Bands 



Origin - 



Figure l.-Electropherogram of starch gel showing general muscle protein patterns of CJi inoei-ftex ha inJi. C. opilio, and hybrids. 



muscle tissue from 10 C. hairdi, 5 hybrids, and 10 

 C. opilio were examined electrophoretically using 

 the methods of Johnson et al. (1972) and the buffer 

 system of Ridgway et al. (1970). 



The electrophoretic patterns of general muscle 

 proteins are shown in Figure 1. All C. opilio 

 patterns possessed a single band (A), while all C. 

 hairdi showed a slower anodally migrating band 

 (B). The five hybrids possessed three bands: A, B, 

 and an intermediate band AB which indicates 

 hybridization between C. bairdi and C. opilio. 



The intermediate band (AB) was less intense 

 than either of the other bands (A or B). A 1:2:1 

 ratio is expected in random combination of 

 dimeric protein. I thus assume that there is non- 

 random association between the protein units. 



Further investigation is needed to determine if 

 the electrophoretic patterns reported here are 

 evident in all possible crosses between the two 

 parent species and that the parental patterns are 

 invariant throughout their ranges. 



Acknowledgments 



I thank Robert J. Wolotira, Jr. (Northwest 

 Fisheries Center, NMFS, NOAA, Seattle, Wash.) 

 for providing identified crab samples for this 

 report. 



Literature Cited 



HooPES, D. T., J. F. Karinen, and M. J. Pelto. 



1972. King and Tanner crab research. Int. North Pac. Fish. 

 Comm., Annu. Rep. 1970:110-120. 

 Johnson, A. G., F. M. Utter, and H. 0. Hodgins. 



1972. Electrophoretic investigation of the family Scorpaen- 

 idae. Fish. Bull., U.S. 70:403-413. 



Karinen,.!. F. 



1974. King and Tanner crab research, 1971. Int. North Pac. 

 Fish. Comm., Annu. Rep. 1972:102-111. 

 Karinen, J. F., and D. T. Hoopes. 



1971. Occurrence of Tanner crabs {Cliionoecetea sp.) in the 

 eastern Bering Sea with characteristics intermediate 

 between C. hairdi and C. opilio. (Abstr.) Proc. Natl. 

 Shellfish Assoc. 61:8-9. 

 Ridgway, G. J., S. W. Sherburne, and R. D. Lewis. 



1970. Polymorphism in the esterases of Atlantic herring. 

 Trans. Am. Fish. Soc. 99:147-151. 



Allyn G. .Johnson 



Northurst Fiftheriex Center 



National Marine Fisheries Service, NOAA 



Seattle, Wash. 



Present address: Gulf Coastal Fisheries Center Port 



Aransas Laboratory, NMFS, NQAA 



West Port Street, Port Aransas, TX 78373 



EFFECTS OF BENZENE ON GROWTH, 



FAT CONTENT, AND CALORIC CONTENT 



OF STRIPED BASS, MORONE SAXATILIS 



The San Francisco Bay area is a major terminus 

 and refinery area for crude oil, and oil-related 

 activities in the area are expected to increase 

 because of the Alaska pipeline and expanded 

 drilling on the outer continental shelves of 

 California and Alaska. The San Francisco Bay- 

 delta region supports a number of fisheries, in- 

 cluding the most important recreational striped 

 bass, Morone saxatilis, fishery on the west coast. 

 Information on the toxicity of aromatics in crude 

 oil to striped bass and other fisheries is needed. 



694 



