SHERBURNE: BLOOD CELLS OF CENTROSCYMNUS COELOLEPIS 



1^ 



10 Microns 

 I 1 



Figure 8. — Neutrophil showing a segmented nucleus. 



Hematocrit 



The packed cell volume of this deepwater 

 shark was less than that reported for other 

 sharks. In a 62 mm whole blood column, 52 

 mm was clear plasma, 2 mm buffy coat, and 

 8 mm packed erythrocytes. This was a 13% 

 hematocrit compared with 17-32% from 11 wild 

 dogfish (Burger, 1967) and 23.1% for the 

 hammerhead shark (Altman and Dittmer, 1961). 



The blood of the Portuguese shark examined 

 showed no evidence of abnormality. There was 

 no hemolysis in the plasma, and though the 

 absolute cell sizes were larger than previously 



reported for other sharks, the staining and 

 morphological characteristics of each cell cate- 

 gory were typical of shark cells. 



ACKNOWLEDGMENT 



I wish to thank Gareth W. Coffin of the 

 Northeast Fisheries Center, Boothbay Harbor 

 Laboratory, National Marine Fisheries Service 

 for the photomicrographic work. 



LITERATURE CITED 



Altman, P. L., and D. S. Dittmer. 



1961. Blood and other body fluids. Fed. Am. Soc. 

 Exp. Biol., Wash., D.C., p. 1 16-119. 



BiGELOW, H. B., AND W. C. SCHROEDER. 



1948. Sharks, hi A. E. Parr and Y. H. Olsen 



(editors), Fishes of the western North Atlantic. 



Part One, p. 59-546. Sears Found. Mar. Res., 



Yale Univ., Mem. 1. 

 Burger, W. J. 



1967. Problems in the electrolyte economy of the 



spiny dogfish, Squaliis acanthias. In P. W. Gilbert, 



R. F. Mathewson, and D. P. Rail (editors). 



Sharks, skates, and rays, p. 177-185. Johns Hopkins 



Press, Bait. 

 Saunders, D. C. 



1966a. Elasmobranch blood cells. Copeia 1966:348- 



351. 

 1966b. Differential blood cell counts of 121 species 



of marine fishes of Puerto Rico. Trans. Am. 



Microsc. Soc. 85:427-449. 



439 



