FISHERY BULLETIN: VOL. 69. NO. 1 



TEMPERATURE 

 30° C 



TEMPERATURE 

 40° C 



TEMPERATURE 

 50°C 



JOOr 



TEMPERATURE 

 60°C 



20 30 40 

 TIME IMIN.) 



TEMPERATURE 

 70° C 



TEMPERATURE 



eo°c 



20 30 40 

 TIME (MIN.) 



Figure 3. — Protein autolysis rates of whole hake (outside) homogenates held at various pH's and temperatures. 



TEMPERATURE 

 30°C 



TEMPERATURE 

 40" C 



TEMPERATURE 

 50° C 



300 



O 100 



10 20 30 40 

 TIME (MINI 



5.0 

 40 

 " 9 



TEMPERATURE 

 70°C 



20 30 40 

 TIME IMIN) 



TEMPERATURE 

 80°C 



20 30 40 

 TIME ( MIN 1 



Figure 4. — Protein autolysis rates of whole hake (inside) honioRenates held at various pH's and temperatures. 



teolytic enzyme in the outside hake taken for 

 this study resides in the tissue. 



EFFECT OF AUTOLYTIC ACTIVITY 

 ON YIELD OF FPC 



MATERIALS AND METHODS 



Whole inside hake wei'e used throughout this 

 experiment. For the preparation of FPC, whole 



hake was comminuted by passing through a Ho- 

 bart grinder (equipped with a i ..-inch plate). 

 Aliquots of the comminuted fish were acidified 

 with 0.1 M HCl to pH 5.5 and 4.5. The original 

 pH of the fish was 6.9. The treated fish was 

 allowed to autolyze at 50° C for 0, 20, 40, and 

 60 min. After each time interval, FPC was pre- 

 l)ared by isopropanol (IPA) extraction as fol- 

 lows: The fish was successively extracted (4 



244 



