KIFER. SMITH, and YOUNG: EFFECT OF DIETARY FISH OIL 



Table 25. — Inner backfat tissue — comparison of correlation coefficients and significant degree of polynomial re- 

 gression obtained by relating the quantity of menhaden oil consumed (A') until the pigs attained a body weight 

 of 90.9, 79.5, or 68.0 kg to the amount of individual fatty acids deposited in inner backfat tissue (Y). 



P <,05 



P <,01 



derived from these acids and in a concomitant 

 decrease in the quantity of acids of the other 

 families. 



In Trial II, we demonstrated the interrela- 

 tion of fatty acid families. 



Specific evidence that the deposition of the cj6 

 and &j9 families are inhibited appears in Tables 

 24 to 27. As the quantity of the uiZ fatty acids 

 fed and deposited increased, the quantity of the 

 20:2 w6, 21:1 aj9, 20:4 w6, and 22:4 co6 fatty 

 acids found in the various tissues decreased 

 significantly. The linolenic (co3) family fatty 

 acids of the menhaden oil therefore inhibited 

 the conversion of oleic (18:1 ttj9) and linoleic 

 (18:2 ojG) to the elongated and dehydrogenated 

 members of their respective families by prevent- 

 ing the addition of two carbons or the removal 

 of two hydrogens, or by preventing both the 

 addition of two carbons and the removal of two 

 hydrogens. 



SUMMARY AND CONCLUSIONS 



Pigs were fed diets containing fish oil in two 

 feeding trials to investigate (1) the organo- 

 leptic effect produced in pig tissue by feeding 

 pigs stabilized crude menhaden oil; (2) the pos- 

 sible retention or disappearance of off-flavor by 

 withdrawing the fish oil from the diet at given 

 times; (3) the nature of the fatty acid compo- 

 sition of the inner backfat tissues, the outer 

 backfat tissues, the liver tissues, and the longis- 

 simus dorsi tissues; (4) the relation of compo- 

 sition to off -flavor (if an off-flavor is produced) ; 

 and (.5) the hyi)othesized metabolic interactions 

 of fatty acid families. 



An off-flavor and a fishy flavor were detected 

 in the meat of pigs fed diets containing about 

 fr of menhaden oil. 



Gas-liquid chromatographic analyses of the 

 tissues indicated that up to 28 saturated and 



299 



