FISHERY BULLETIN: VOL. 69, NO. 



Methyl esters of fatty acids taken from the 

 various tissues were analyzed with an F&M Bio- 

 medical Model 400 gas chromatograph. The in- 

 strument was equipped with a hydrogen flame 

 detector. The column used was comi)osed of 4.0- 

 mm ID by 243.8-cm Pyrex glass containing 5.0'^ r 

 (by weight) of diethylene glycol succinate poly- 

 ester (DEGS from Wilkens Instrument and Re- 

 search Inc.) supported on 80- to 90-mesh acid- 

 base washed and siliconized flux-calcinated 

 diatomaceous eai-th (Anakron ABS). The op- 

 erating conditions were as follows: column 

 temperature, 165° C; flash-heater temperature, 

 285° C; detector temperature, 200° C; and in- 

 itial attenuation that corresponds to 10 to 14 



amp full-scale deflection. The inlet pressure 

 of the column measured 40 psi of helium, the 

 flow measured 53 ml per min at the outlet of 

 the column. The size of the injected sample was 

 about 0.12 juliter. 



The area-percent method was used to deter- 

 mine the corresponding peak areas of the curves 

 obtained from the gas-liquid chromatographic 

 recorder. The fatty acid composition (in aver- 

 age percentage) of each sample was calculated 

 by multiplying peak height by retention time 

 and then multiplying this product by 100 and 

 dividing by the total area. 



Certain diff'erences were obtained in the total 

 degree of saturation and quantity of specific 



Table 5. — Summary of gas-liquid cliromatographic analyses indicating comparative degree of unsaturation and 

 quantity of selected fatty acids within and among the tissues obtained from pigs fed either 0% or 1.47c dietary 

 menhaden oil. 



Type of fatty octd 



Concentration of the vorious fatty acids in the vorious tissues 

 when the relative amount of menhaden oil in the diets was: 



0% 



Backfat 



Inner 

 tissue 



Outer 

 tissue 



Longijiimul 

 dorsi tissue 



Liver 

 tissue 



1 .4% 



Backfat 



Inner 

 tissue 



Outer 

 tissue 



Longiisimus 

 dorti tissue 



Liver 

 tissue 



Note: The equivalent degree of unsaturation In the fatty adds was obtained by multiplying the number of double bonds by the quantity of fatty acid. 



286 



