FISHERY BULLETIN: VOL. 73, NO. 2 



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15 



counted and measured in the laboratory. The Card 

 Sound samples from the four stations were pooled 

 for each net on each sampling date in proportion to 

 the filtered volumes they represented (Reeve and 

 Cosper 1973). Aliquots of each pooled sample were 

 taken such that they contained between 50 and 100 

 organisms. The total body length of each animal 

 was measured (see Reeve 1970). The entire sample 

 was examined for mature animals. The lengths 

 were tabulated in 1-mm preserved length size 

 classes (see next section for conversion to live 

 length). Since two values were obtained for each 

 size class from the Card Sound samples (i.e., one 

 for each mesh size) the larger number was taken as 

 the correct one, on the assumption that the smaller 

 value was due either to avoidance by larger 

 animals of the 64-/Am mesh, or escape of the 

 smaller animals through the 200-/i,m mesh. 



The pooled 200-/Am Biscayne Bay samples were 

 treated similarly. The numbers of S. hispida in 

 Biscayne Bay were estimated by adjusting the 

 numbers in each size class in the 200-/im net to 

 total number on the basis of ratios computed for 

 the 64- and 200-/xm counts from Card Sound. 



Analysis of ctenophore samples from the 1-m 

 net presented special difficulties, because there 

 was no known satisfactory method of preservation 

 of lobate ctenophores. Following Miller (1970) 

 analysis was performed on deck immediately after 

 recovery of the net (see Baker 1973). The contents 

 of the cod end were emptied into a stack of wire 

 sieves of arbitrarily chosen decreasing mesh sizes 

 (25-, 12.5-, 6.25-, 3.0-, and 0.7-mm mesh openings) 

 immersed in seawater. The ctenophores from each 

 sieve, except the smallest, were transferred to a 

 graduated cylinder and the total volume of or- 

 ganisms retained by each sieve measured. The 

 average volume per individual retained in each 

 sieve was determined either by counting the total 

 number of animals in each sieve or, in the case of 

 the larger animals, by direct volume displacement 

 of randomly selected individual ctenophores. It 

 was impractical to follow this routine with the 

 smallest animals (0.7-mm sieve) since their total 

 volume was too small to be measured accurately. 

 Instead, they were resuspended in seawater, 

 transferred to plastic bags, and returned to the 

 laboratory where they were counted. No attempt 

 was made to assess the number and hence produc- 

 tion of ctenophores smaller than 0.7 mm in 

 diameter. 



240 



