FISHERY BULLETIN; VOL. 72. NO. 2 



RETENTION TIME IN MINUTES 



Figure 1 1. — Chromatogram of DDT analog standard and of a fat 

 sample from Sebastes paucispinis taken in Santa Monica Bay 7 

 January 1972. p.p'DDE (98 ppm) is off scale. Following cessation 

 of dumping of DDT wastes and flushing out of sewer lines in 1970, 

 p.p'DDD (15 ppm) has exceeded p,p' DDT (6.1 ppm) in most fish 

 specimens tested. Prior to cessation of dumping and flushing of 

 sewer lines, DDT was almost always present in greater quantities 

 than DDD. 



Point plant and out into the ocean. Sewer water 

 from these deposits contained 48% DDD as op- 

 posed to 2-6% in the original Montrose discharges, 

 and although the total amount of DDT and its 

 metabolites was much less than before April 1970, 

 the total amount of DDD entering the ocean ap- 

 peared to be several times greater than it had been 

 before the dumping stopped in April. This would 

 account for the increase in DDD in the myctophids 

 taken in 1972 rather than the expected decrease 

 indicated by the calculated line (Figure 10, Table 

 1). A mud sample taken from the ocean floor a few 

 miles from the sewer outfall in July 1971, just 

 after the sewer cleaning operations ceased con- 

 tained 6%DDT, 82% DDE, and 12% DDD (Figure 

 12). This compares favorably with the myctophids 

 taken in April 1972, 9:79:12, and the S. pauci- 

 spinis fat samples (Figure 11) taken in January 

 1972, 5:79:16, and indicates that the fish reflect 

 the values of these analogs in the environment 

 fairly well. 



8 9 10 

 RETENTION TIME IN MINUTES 



Figure 12. — Chromatogram of DDT analog standard and sample 

 of mud from the ocean floor in the Los Angeles area taken in August 

 1971, 16 mo after most dumping of DDT wastes stopped. DDD 

 greatly exceeds DDT. This may have resulted from the sewer 

 cleaning operations, or it may have been the condition existing 

 before and merely reflect what the biota can excrete more easily. In 

 the Sebastes chromatogram (Figure 11), the o.p'DDE peak is 

 within the limits of the right proportions top.p'DDE for it to be 

 considered o.p'DDE. In the mud sample it is much too high and 

 may be DDMU (a metabolite of DDD) which has the same reten- 

 tion time on this column as o.p'DDE. 



The most noticeable difference between the pes- 

 ticide metabolites in the fish (Figure 11) and the 

 mud (Figure 12) were the two prominent peaks 

 preceding p.p'DDE. The peak at the locus of 

 o,p'DDE also may contain DDMU, a metabolite of 

 DDD. The other peak could be a metabolite of 

 Kelthane. However, several dozen additional mud 

 samples tested subsequently did not contain these 

 peaks except for expected amounts of o,p 'DDE. 

 The mud sample (Figure 12) was run while we 

 were experimenting with methods of determining 

 pesticide content of the mud samples. The subse- 

 quent samples were run after we had settled on a 

 different method that gave maximum recovery of 

 DDT, DDD, and DDE without special regard to 

 other CHC. These subsequent mud samples 

 yielded chromatograms almost identical with 

 those offish and other biological samples from the 

 same general area. 



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