FISHERY BULLETIN: VOL. 72. NO. 3 



and Prasad, 1970), naphthyl acetate and pro- 

 pionate esterases (modified after Utter, Stormont, 

 and Hodgins, 1970), and general protein (John- 

 son et al., 1972). 



RESULTS AND DISCUSSION 



Bands of identical electrophoretic mobility for a 

 given enzyme system were observed in all species 

 for the follov^fing systems: MDH (NADP), one 

 anodal band; MDH (NAD), one anodal band; 

 peptidase (valyl-leucine), three anodal bands; TO, 

 one anodal band; and GAPDH, three anodal 

 bands. 



Esterase patterns varied among species; how- 

 ever, the patterns were weak and not completely 

 repeatable. Gasser and Rowlands (1972) noted 

 similar problems in interpreting esterase patterns 

 from human serum and related the differences to 

 nongenetic causes. We have, therefore, excluded 

 them from further consideration in this study. 

 Esterases have been found useful in studies of 

 other invertebrates and may indeed be of use in 

 studies of pandalid shrimp if reliable methods 

 can be developed for stabilizing expression of 

 patterns (Manwell and Baker, 1970; Barlow and 

 Ridgway, 1971). 



Lactate dehydrogenase was expressed as one 

 anodal band — with a broad, faint-staining area 

 anodal to it — in each species. The bands of 

 P. jordani, P. borealis, and P. goniurus had an 

 identical mobility slightly more anodal than the 

 bands of P. hypsinotus and Pandalopsis dispar. 



The general protein patterns observed are 

 shown in Figure 1. Pandalus hypsinotus had 



bands C, E, F, and G; P. jordani and P. borealis, 

 bands A, B, D, and G; and P. goniurus and 

 Pandalopsis dispar, bands A, B, and G. The B 

 band, although qualitatively invariable, varied 

 considerably in intensity in all species expressing 

 it. A greater degree of difference in the protein 

 pattern was observed between Pandalus hyp- 

 sinotus (processing three unique bands) and the 

 other four species than was observed among 

 these four species. 



Phosphoglucomutase (PGM) was polymorphic 

 in all five species. Two-banded phenotypes (Figure 

 2) were observed in some individuals of all 

 species, presumably reflecting heterozygous 

 individuals, and the pattern suggests that the 

 active PGM enzyme in shrimp is a monomer 

 (Shaw, 1964). This agrees with reports of PGM 

 polymorphisms found in vertebrates (see Johnson, 

 Utter, and Hodgins, 1971). A diagrammatical 

 representation of the allelic forms found within 

 the five species (Figure 3) shows the six allelic 

 bands that were observed and designated (in 

 decreasing anodal mobility) A, B, C, D, E, and F. 

 The distribution of these alleles, as indicated in 

 Figure 3, was P. hypsinotus, A, B, E, and F; 

 P. goniurus, A and B; P. borealis, C, E, and F; 

 P. jordani, B, C, and E; and Pandalopsis dispar, 

 C, D, and E. The phenotypic distributions of 

 PGM in the five species of shrimp along with gene 

 frequencies, Hardy-Weinberg calculations and 

 collections data are presented in Tables 1 and 2. 

 With the exception o{ Pandalus hypsinotus , the 

 phenotypic distributions of PGM of the collections 

 of shrimp species did not deviate significantly 



-A 

 B 



■c 



r-G 



— Upper 

 Boundary 



+ 

 a 



— ^*^^ -"■«#»' 



Origin 



12 3 4 5 



12 3 4 5 



Figure 1. — Electrophoretic patterns on starch gel of muscle 

 protein of five species of shrimp from the northeastern Pacific 

 Ocean. Numbers below the patterns indicate the following 

 species: 1. Pandalus jordani, 2. P. borealis, 3. P. goniurus, 

 4. Pandalopsis dispar, and 5. Pandalus hypsinotus. 



Origin . 

 Phenotypes 



CE CC CE CC BC 



CE CC CE CC BC 



Figure 2. — Phosphoglucomutase phenotypes of Pandalus 

 jordani in starch gels suggesting monomeric configuration of 

 this enzyme. 



800 



