FISHERY BULLETIN: VOL. 70, NO. 2 



Table 1. — Location and number of specimens of Scor- 

 paenidae collected, 1968-70. 



Location^ Total 



Species  — number 



ABGDEFof fish 



Pacific Sibastes 



S. aleutianus 10 — 6 — -- — 16 



S. alutus 217 - 843 .. „ — 1,060 



S. auriculatus — 76 — — — — "76 



S. aurora 3 — — — — — 3 



S. br^vispinis 5 — 40 „ — — 45 



S. catnaematicus — — — 3 3 



S. caurinus .. 283 — — — — 283 



S. chlorostictu! — — — 1 — ' 



S. crameri 2 — 16 — — — 18 



S. diploproa 14 — — — — — 14 



S. tlongatui .. 297 96 .. .. - 393 



S. entomilas 2 — 2 ._ — — 4. 



S. flavidus 8 — — — — — 8 



S. helvomaculatus 5 .. 19 .. ._ ._ 24 



S. levii — — — — 1 — 1 



S. malisfr .. 25 .. ._ — — 25 



S. milanops __ 28 — — — — 28 



S. paucispinis 2 15 — 1 — 18 



S. pinniger „ — 24 .. ._ __ 24 



S. proriger 9 — 100 .. - - 109 



S. reedi 1 — 110 .. - - HI 



S. rvbtrrimus 5 27 5 .. — — 37 



S. rubivinctus 5 -- 34 „ ._ -- 39 



S. saxicola 5 — -- ._ 1 _- 6 



S. vjihoni — 1 — — — ' 



S. variegatus — — — — 1 ' 



S. zacentrus 1 — 37 — — — 38 



Atlantic Sfbastes 



S. marinus — 9 — — 9 



S. viviparous — 10 — — 10 



Sebaitolobus 



alascanus „ — 100 ._ — — 100 



Heticottnus 



dactylopterus -. — — 10 — — 10 



1 A = Pacific Coast of Washington and Oregon, 1968-70; B = Puget 

 Sound, Wash., 1968-70; C = Queen Charlotte Sound, B.C., Canada, June 



1970; D = West Coast of Britain and Ireland, August 1970; E = Avila 



Beach, Calif., October 1970; F = Cape Ommaney, Alaska, April 1970. 



volumes of tissue and phosphate-buffered physi- 

 ological saline solution (pH 7.4) into uniform 

 pastes with glass rods. The extracts were tested 

 by electrophoresis without further treatment by 

 (1) drawing them into 1/4,-inch X 3/16-inch filter 

 paper inserts (Schleicher and Schuell grade S 

 and S No. 470)', placed on the surface of the tis- 

 sue-saline mixture, and (2) placing the inserts 

 into starch gels. 



Electrophoresis in starch gel followed the 

 methods of Kristjansson (1963). All but two 

 of the biochemical systems were resolved well 

 using a buffer system described by Markert and 

 Faulhaber (1965). Lactate dehydrogenase and 



' Reference to trade names in this publication does not 

 imply endorsement of commercial products by the Na- 

 tional Marine Fisheries Service. 



phosphoglucomutase were best resolved by using 

 the buffer system described by Ridgway, Sher- 

 burne, and Lewis (1970) . Gels consisted of 35 g 

 starch plus 250 ml of buflfer. A voltage of 300 

 was applied for 10 min; sample inserts were 

 removed and 400 v applied until indicator dye 

 markers reached a point 6 to 9 cm anodal to the 

 origin. The gels were cooled during electro- 

 phoresis by placing ice packs on glass plates on 

 top of the gels. After electrophoresis, bands re- 

 flecting enzyme activity were detected by the fol- 

 lowing methods: 



Tetrazolium oxidase (TO) (after Brewer, 1967, 

 and Johnson, Utter, and Hodgins, 1970b) : 

 5 mg phenazine methosulfate (PMS) 

 3 mg p-nitro blue tetrazolium (NET) 

 40 ml tris-citrate buffer (0.03 M tris, 0.005 M 

 citric acid, pH7.0) 



L-alpha-glycerophosphate dehydrogenase 



(aGPDH) (after Nyman, 1967, and Johnson, 

 Utter, and Hodgins, 1970a) : 

 5 mg PMS 

 3 mg NET 

 5 mg NAD + 



100 mg L-alpha-glycerophosphate 

 40 ml tris-citrate buffer 



Lactate dehydrogenase (LDH): 

 10 mg PMS 

 5 mg NET 

 5 mg NAD + 



20 ml of 0.5 M sodium lactate solution 

 40 ml tris-citrate buffer 



Peptidase A (after Lewis and Harris, 1967, and 

 Lewis and Truslove, 1969) : 



10 mg DL valyl-leucine 



1 mg horseradish peroxidase 



5 mg 0-dianisidine in 10 ml acetone 



0.5 ml M MgCla 



1 mg Bothrops atrox venom 



40 ml tris-citrate buffer 

 Phosphoglucomutase (PGM) (after Spencer, 

 Hopkinson, and Harris, 1964) : 



100 mg glucose-1-phosphate (dipotassium 

 salt) 



5 mg NADP 



5 mg PMS 



3 mg NET 



20 units glucose-6-phosphate dehydrogenase 



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