JOHNSON, UTTER and HODGINS: ELECTROPHORETIC INVESTIGATION 



1 Modified after Tsuyuki et ol., 1968. 



a Species with unique biological characteristics. 



^ Pattern of the single specimen tested has not been described. 



0.5 ml 1 M MgCl2 



40 ml tris-citrate buffer 



Isocitrate dehydrogenase (ICDH): 



(a) NADP dependent (after Opher, Leonard, 



and Miller, 1969): 

 30 mg DL sodium isocitrate 

 5 mg NADP 

 0.5 ml 1 M MgCl2 

 5 mg PMS 

 5 mg NBT 

 40 ml tris-citrate buffer 



(b) NAD + dependent: 



Same formulation as NADP dependent, 



but substituting 10 mg NAD + for 5 mg 



NADP 



Muscle protein detected by nonspecific protein 



staining using 1% nigrosin-buffalo black in 



solution of 1:4:5 acetic acid: methanol: water 



and destained with a 1:4:5 solution of acetic 

 acid, methanol, and water. 



ENZYME AND PROTEIN PHENOTYPES 

 TETRAZOLIUM OXIDASE (TO) 



Interspecific variation of TO was previously 

 reported in the genus Sebastes (Pacific) by 

 Johnson et al. (1970b), where three anodal mo- 

 bilities were observed in 15 species studied: 

 Fast (F),Slow (S), and Very Slow (VS). These 

 findings are expanded in the present study 

 (Table 2, Figure 1). The F band occurred in 

 15 of the 27 Pacific Sebastes species; the S band 

 was present in 9 species and the VS band in 3 

 species. Only the S band occurred in both At- 

 lantic Sebastes species. The VS band was found 



405 



