FISHERY BULLETIN: VOL. 70, NO. 2 



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Figure 1. — Band in starch-gel illustrating the four tetrazolium oxidase phenotypes, F, 

 S, S-VS, and VS, detected in the family Scorpaenidae. The following samples are shown: 

 1, 5 Helicolenus dactylopteriis (S-VS), 2 Sebastes reedi (VS), 3 Sebastes caurinus (F), 

 4 Sebastes alutus (S), and 6 Sebastes reedi (VS). 



in Sebastolobus alascanus.' Helicolenus dactyl- 

 opteriis was polymorphic for the S and VS bands; 

 of the 10 samples tested, two exhibited a three- 

 banded phenotype having the S and VS bands 

 in addition to another band of intermediate mo- 

 bility, whereas the rest had only the single S 

 band. The three-banded phenotype suggests 

 that two TO alleles are segregating in Helicole- 

 nus and that tetrazolium oxidase functions as a 

 dimer in scorpaenids. This interpretation is 

 consistent with TO polymorphisms observed in 

 salmonids (Utter, 1971) where three-banded 

 phenotypes were observed in heterozygous rain- 

 bow trout (Salmo gairdneri) and chinook salmon 

 (Oncorhynchus tshaivytscha) . 



L-ALPHA -GLYCEROPHOSPHATE 

 DEHYDROGENASE (aGPDH) 



Evidence for a polymorphic dimer having two 

 alleles — Fast (F) and Slow (S) — were described 



* We used liver extracts of this species for detection 

 of TO activity because muscle extracts failed to develop 

 TO bands. We assume that this is a valid comparison 

 because of parallel TO activity between liver and muscle 

 observed in other scorpaenid species. All other scorpae- 

 nid enzymes tested were extracted from skeletal muscle. 



in S. alutus (Johnson et al,, 1970a) . In addition 

 to the F and S bands, three faster aGPDH bands 

 have been observed among the scorpaenids that 

 we have tested: E, D, and C,° listed according 

 to increasing mobility (Figure 2 and Table 2). 

 Additional aGPDH bands invariably occurred, 

 regardless of phenotype, when electrophoresis 

 proceeded beyond a 6-cm anodal migration of the 

 dye marker. These bands are presumably arti- 

 facts of electrophoresis and did not alter our in- 

 terpretation of enzyme variations. This phe- 

 nomenon was also noticed by McCabe, Dean, and 

 Olson (1970) in aGPDH variants of skipjack 

 tuna (Katsmvomis pelamis) . 



In Pacific Sebastes, 19 species were monomor- 

 phic for the E band. S. auricukitus was poly- 

 morphic for the E and F bands. S. caurinus as 

 well as S. alutus were polymorphic for F and S 

 bands. S". maliger and S. saxicola were mono- 

 morphic for the F band. In the Atlantic Se- 

 bastes, S. viviparous was monomorphic for the 

 E band and S. mar inns was polymorphic for the 

 E and F bands. The D and C bands were mon- 

 omorphic Sebastolobus alascanus and Helicole- 

 nus dactylopterus, respectively. 



^ The separation of aGPDH bands C and D depends 

 on optimal electrophoretic conditions. 



406 



