JOHNSON, UTTER and HODGINS: ELECTROPHORETIC INVESTIGATION 



-L therefore called these regions peptidase A-I and 

 i peptidase A-II. 



iggjg^ 



Origin 



I 



8 



Figure 2. — Bands in starch-gel illustrating four L-alpha 

 glycerophosphate dehydrogenase phenotypes (C, D. E, 

 F) detected in the family Scorpaenidae. The following 

 samples are shown: 1, 5 Helicolenus dactylopterus (C), 

 2, 6 Sebastolobus alascanus (D), 3, 7 Sebastes rubri- 

 vinctus (E), and 4, 8 Sebastes alutus (F). 



LACTIC DEHYDROGENASE (LDH) 



Muscle LDH was resolved as a single anodal 

 band in each scorpaenid species we tested. This 

 agrees with studies of Wilson, Kitto, and Kaplan 

 (1967), who found single anodal bands of mus- 

 cle LDH in two scorpaenid species, Sebastes ma- 

 rinus and Scorpaenopsis gibbosa. The electro- 

 phoretic mobilities were distinct in each species. 

 LDH bands of three different mobilities (A, B, 

 and C) were found in our sampling (Figure 3 

 and Table 2) . No poljonorphisms were detected. 

 All but two Pacific Sebastes species expressed 

 the C band. The B band was found in S. helvo- 

 maculatiis and S. chlorostictus. The B band was 

 found in two Atlantic Sebastes species and Heli- 

 colenus dactylopterus. Only Sebastolobus alas- 

 canus expresses the LDH A band. 



PEPTIDASE 



Peptidase staining occurred in two anodal re- 

 gions for all species tested (Figure 4, Table 2). 

 Both regions are developed with the dipeptide 

 valyl-leucine, which is the specific substrate for 

 peptidase A in mammals (Lewis and Harris, 

 1967; Lewis and Truslove, 1969). We have 



+ 



I— Origin 



Figure 3. — Bands in starch-gel illustrating the three 

 phenotypes of lactate dehydrogenase detected in the fa- 

 mily Scorpaenidae. The following species are shown: 

 1, 4 Sebastolobus alascanus (A), 2, 5 Sebastes helvomac- 

 tilatus (B), and 3, 6 Sebastes alutus. 



Five diflferent bands (a, b, c, d, e) were ob- 

 served in the peptidase A-I (fast) zone. In Pa- 

 cific Sebastes, peptidase A-I bands were ex- 

 pressed as follows: P - S. chlorostictus, S. levis, 

 and S. variegatus; P - S. caurinus, S. auricula- 

 tus, and S. maliger. S. marinus had band P as 

 did 9 of the 10 S. viviparous tested; Sebastolobus 

 alascanus had band P ; and H. dactylopterus had 

 band P. The aberrant Sebastes viviparous sam- 

 ple had a single P band but corresponded to S. 

 viviparous in all other systems tested. The sig- 

 nificance of the variant is unclear. It may re- 

 flect an intraspecies genetic variant (although 

 multiple bands would be expected if this were 

 the case) or perhaps a sibling species. Because 

 only muscle samples were available for Atlantic 

 Sebastes, identification of subtle morphological 

 differences between individuals was not possible. 



Bands of five different mobilities (a, b, c, d, e) 

 were also observed in the peptidase A-II (slow) 

 zone. Band IP was expressed in all but two Pa- 

 cific Sebastes tested; band IP was found in S. 



407 



