JOHNSON, UTTER and HODGINS: ELECTROPHORETIC INVESTIGATION 



tested for some species that were listed as mon- 

 omorphic are too few to preclude the possibility 

 of polymorphism. 



ISOCITRATE DEHYDROGENASE, 

 NADP DEPENDENT (ICDH NAD?) 



We tested for both NAD- and NADP-depen- 

 dent ICDH in the 31 species studied and found 

 activity only for the latter form. It is assumed 

 that this represents cytoplasmic ICDH activity 

 (Opher et al, 1969). Two anodal mobilities of 

 ICDH were detected: the band of H. dactylop- 

 terus migrated slightly faster than the band of 

 the other species (Figure 6). No activity was 

 detectable in extracts of Sebastolobus alascanus. 

 Activity was highly labile in all species, requir- 

 ing testing on the same day that the extraction 

 was made. It may be that S. alascanus has an 

 even more labile form of ICDH than the other 

 species tested. 



Table 3. — Phosphoglucomutase phenotypes in muscle 

 samples from species of Scorpaenidae.^ 



+ 

 i 



Origin- 



2 3 4 5 



^ PGM in our samples of S. diploproa and S. viitsoni did not develop. 



Figure 6. — Isocitric dehydrogenase (NADP dependent) 

 bands found in the family Scorpaenidae. Samples 1, 3, 

 5 are Sebastes alutus and samples 2, 4 are Helicolenus 

 dactylopterus. 



MUSCLE PROTEIN 



A satisfactory separation of muscle protein 

 bands was obtained by permitting the dye mark- 

 er to migrate 9.0 cm anodally from the origin. 

 These bands were separated into two regions — 

 A and B (Figure 7). 



Distinct protein patterns occurred in region A, 

 which differ between genera as well as within 

 the genus Sebastes (Pacific) (Table 4). S. 

 aurora has a unique pattern (bands 1, 4) which 

 differed from the other Pacific Sebastes species 

 (bands 1,3). The intergeneric differences in re- 

 gion A were: Sebastes (Pacific) — bands 1, 4 

 and 1, 3; Sebastes (Atlantic) — bands 2, 6; Heli- 

 colenus — bands 3, 7; and Sebastologus — 5, 7. A 

 band (X) which migrated more anodally than 

 band 7 was found in some Sebastes alutus. We 

 assume this band (X) to be an artifact as it did 

 not appear in repeated tests. The most anodal 

 band (8) was found in all samples tested. Cor- 

 responding region A patterns were not described 

 by Tsuyuki et al. (1968) in instances where the 

 same species were tested and may arise from 

 differences in methodology such as buffer sys- 

 tems (Rasmussen, 1969). 



409 



