Genus 



FISHERY BULLETIN: VOL. 70, NO. 2 



Table 4. — Intergeneric comparison of muscle protein bands of Scorpaenids. 



Protein bands 



Subgroup^ 



Region A 



1 2 3 



7 8 



Pacific Stbastes 

 Pacific Stbastes 

 Pacific Stbastes 

 Atlantic Sebastes 

 Sebastolobus 

 Helicolenus 



+ + + 

 + + + 



+ + - + 



+ + 



+ 



+ + + 



-I- + - + + 



+ -. + + - + 



- + .. + + 



+ + + 



1 Pacific Sebastes subgroups after Tsuyuki et al., 1968. 

 " Alphabetical classification after Tsuyuki et al., 1968. 



i 



8 



Region 

 -t A 



-I 



" ^ ^/g ffi 3iaM 



Origin — 



2 3 



■^k+l 



h 



f Region 



B 



lenus. Bands b and c stained weakly in our gels 

 and failed to show in some individuals (Figure 

 7). The slowest anodal bands were f and h 

 which occurred only in S. aurora. Band i oc- 

 curred in all species tested except S. aurora, 

 S. elongatus, and S. entomelas. On the other 

 hand, S. entomelas and S. elongatus were the 

 only species having the j band, bands j and k 

 being polymorphic in S. elongatus (first reported 

 by Tsuyuki et al., 1968). Band k was present 

 in all genera but Sebastolobus, which — in turn — 

 was the only genus expressing band 1. Simi- 

 larly, bands m and p — present in other genera — 

 were absent in Sebastolobus, which uniquely ex- 

 pressed band o. Our methods were unable to 

 detect band q, reported by Tsuyuki et al. in At- 

 lantic Sebastes and Sebastolobiis. 



12 3 4 5 



I Region A (enlarged ) 



Figure 7. — Muscle protein bands in starch-gel: 1 Heli- 

 colenus dactylopterus (A-3, 7), 2 Sebastolobus alascanus 

 (A-5, 7), Sebastes marinu^ (A-2, 6), 4 Sebastes aurora 

 (A-1, 4), and 5 Sebastes alutus (A-1, 3). 



The protein patterns in region B were similar 

 to those described by Tsuyuki et al. (1968) , who 

 described 16 bands (a-p) that varied between 

 genera and species. Three cathodally migrating 

 bands (a, b, c) occurred in Pacific Sebastes (ex- 

 cept S. aurora), Atlantic Sebastes, and Helico- 



COMPARISON OF VARIATION 

 BETWEEN GENERA 



A comparison of the total variation between 

 genera suggests some possible relations. The 

 greatest similarity was between the Pacific Se- 

 bastes and Atlantic Sebastes where all the elec- 

 trophoretic patterns of the Atlantic Sebastes 

 were found in one or more species of the Pacific 

 Sebastes, except for the protein bands of region 



A. Pacific Sebastes and Sebastolobus exhibited 

 common bands for PGM, TO, peptidase A-I, and 

 protein B-i. Pacific Sebastes and Helicolenus 

 shared common bands for LDH, PGM, and pro- 

 tein bands of region B. Helicolenus and one spe- 

 cies of Pacific Sebastes possessed a common pep- 

 tidase A-II band. Helicolenus and Sebastes had 

 common bands in LDH, PGM, and protein region 



B. Helicolenus and Sebastolobus shared only pro- 



410 



