



30 



Microns 



Figure 240. — Amoebocytes (granular cells) of C. virginica observed in vivo. Camera lueida drawings of live cells on glass. 



Figure 241. — Intervascular blood clot and infiltration of amoebocytes in the mantle of green C. virginica. Bouin, 



hematoxylin-cosin. 



vestigators with somewhat different results. Koll- 

 mann (1908) found that marine hmiellibranchs 

 have acidophilic granules, while those of fresh 

 water moUusks are amphophilic. The granules of 

 0. eihdw (Takatsuki, 1934a) are neutrophilic with 

 a tendency to become stained vitally by basic 



CIRCULATORY SYSTEM AND BLOOD 



dj-es. The amoebocytes of C. circumjncfa (Ohuye, 

 1938) liave eosinophilic or amphopliilic cytoplasm 

 and basopiiilic granules. In a blood smear 

 preparation of C. virginica examined in the 

 Bureau's shellfish laboratory the granules stained 

 reddish-purple to dark blue with polyclirome 



263 



