254 



Fishery Bulletin 101(2) 



are some spawning populations that we have not yet ana- 

 lyzed. These populations include those in the Driftwood 

 River and Middle River in the Stuart River drainage, and 

 the Chilako River and Nadina River in the Nechako River 

 drainage. These populations are likely most similar to the 

 Stuart River and Nechako River populations with respect 

 to microsatellite variation, and the Stuart-Nechako stock 

 in the baseline may be a proxy for the occurrence of one or 

 more of these populations in the fishery samples. 



The application of microsatellite variation to estimation of 

 stock composition of chinook salmon in Fraser River mixed- 

 stock fisheries was conducted as a result of conservation 

 concerns, particularly for early-migrating populations. The 

 mixed-stock analysis enabled accurate estimates of stock 

 composition in mixed-stock fishery samples and can even 

 be applied to nonretention fisheries because the fish can be 

 released alive after sampling. The applications developed 

 for Fraser River chinook salmon is an example of the power 

 of microsatellite variation that will likely be applied to an 

 increasing number of species and fisheries for which the 

 management concerns of identifying population structure 

 and detecting specific populations in mixed-stock fisheries 



Acknowledgments 



We would like to acknowledge all those people involved 

 in sampling the commercial fisheries in the Fraser River, 

 with most of the sampling coordinated by Melanie Sullivan 

 and Terry Robertson. The test fishery was conducted and 

 sampled by Alan Baker Improvements to the manuscript 

 were suggested by an anonymous referee. Funding was 

 provided by the Department of Fisheries and Oceans. 



Literature cited 



Banks, M. A., M. S. Blouin, B. A. Baldwin, V. K. Rashbrook, 

 H. A. Fitzgerald, S. M. Blakenship, and D. Hedgecock. 



1999. Isolation and inheritance of novel microsatellites in 

 chinook salmon (Oncorhynchus tachawytscha) . J. Hered. 

 90:281-288. 



Banks, M. A., V. K. Rashbrook, M. J. Calavetta, C. A. Dean, and 

 D. Hedgecock. 



2000. Analysis of microsatellite DNA resolves genetic 

 structure and diversity of chinook salmon {Oncorhynchus 

 tshawytscha) in California's Central Valley. Can. J. Fish. 

 Aquat. Sci. 57 915-927. 



Beacham, T D., J. R. Candy, K. J. Supemault, T. Ming, B. Deagle, 

 A. Schulze, D. Tuck, K. H. Kaukinen, J. R. Irvine, K. M. Miller, 

 and R. E. Withler. 



2001. Evaluation and application of microsatellite and major 

 histocompatibility complex variation lor stock identification 

 of coho salmon in British Columbia. Trans. Am. Fish. Soc. 

 130:1116-1149. 



Beacham, T. D, K. D. Lc. M. R. Raap, K. Hyatt, W. Luedke, and 

 R. E. Wither 

 2000. Microsatellite DNA variation and estimation of stock 

 composition of Barkley Sound. British Columbia sockeye 

 salmon. Fish. Bull. 98:14-24. 



Beacham, T. D., and C. B. Murray. 



1993. Fecundity and egg size variation in North American 

 Pacific salmon {Oncorhynchus). J. Fish Biol. 42:485-508. 

 Beacham, T. D., S. Pollard, and K. D. Le. 



1999. Population structure and stock identification of steel- 

 head in southern British Columbia, Washington, and the 

 Columbia River based on microsatellite DNA variation. 

 Trans. Am. Fish. Soc. 128:1068-1084. 

 Beacham, T. D., K. J. Supemault, M. Wetklo, B. Deagle, 



K. Labaree, J. Irvine, J. R. Candy, K. M. Miller, R. J. Nelson, 

 and R. E. Withler 

 2003. The geographic basis of population structure of Fraser 

 River chinook salmon {Oncorhynchus tshawytscha). Fish. 

 Bull. 101:229-242. 

 Beacham. T. D., R. E. Withler, and T A. Stevens. 



1996. Stock identification of chinook salmon {Oncorhynchus 

 tshawytscha) using minisatellite DNA variation. Can. J. 

 Fish. Aquat. Sci. 53:380-394. 

 Beacham, T. D, and C. C. Wood. 



1999. Application of microsatellite DNA variation to esti- 

 mation of stock composition and escapement of Nass River 

 sockeye salmon {Oncorhynchus nerka). Can. J. Fish. 

 Aquat. Sci. 56:1-14. 

 Buchholz, W., S. J. Miller and W. J. Spearman. 



1999. Summary of PCR primers for salmonid genetic studies. 

 U.S. Fish. Wild. Serv. Alaska Fish. Prog. Rep. 99-1, 30 p. 



Candy, J. R, and T. D. Beacham. 



2000. Patterns of homing and straying in southern British 

 Columbia coded-wire tagged chinook salmon {Oncorhyn- 

 chus tshawytscha) populations. Fish. Res. 47:41-56. 



Cornuet, J. M., S. Piry, G. Luikart, A. Estoup, M. Solignac. 



1999. Comparison of methods employing multilocus geno- 

 types to select or exclude populations as origins of indi- 

 viduals. Genetics 153:1989-2000. 



Debevec, E.M., R. B. Gates, M. Masuda, J. Pella, J. Reynolds, and 

 L. W. Seeb. 



2000. SPAM (version 3.2): Statistics program for analyzing 

 mixtures. J. Hered. 91:509-510. 



Fraser, F J., P. J. Starr, and A. Y. Federenko. 



1982. A review of the chinook and coho salmon of the Fraser 

 River Can. Tech. Rep. Fish. Aquat. Sci. 1126, 130 p. 

 Miller, K. M., R. E. Withler, and T. D. Beacham. 



1996. Stock identification of coho salmon (Oncorhynchus 

 kisutch) using minisatellite DNA variation. Can. J. Fish. 

 Aquat. Sci. ,53:181-195. 

 Murray, C. B., and M. L. Rosenau. 



1989. Rearing of juvenile chinook salmon in nonnatal tribu- 

 taries of the lower Fraser River, British Columbia. Trans. 

 Am. Fish. Soc. 118:284-289. 

 Nelson, R. J., and T. D. Beacham. 



1999. Isolation and cross species amplification of microsat- 

 ellite loci useful for study of Pacific salmon. Anim. Genet. 

 30:228-229. 

 Nelson, R. J., T. D. Beacham, and M. P. Small. 



1998. Microsatellite analysis of the population structure of 

 a Vancouver Island sockeye salmon {Oncorhynchus nerka) 

 stock complex using non-denaturing gel electrophoresis. 

 Mol. Mar Biotech 7: 312-319. 

 Nelson, R. J., M P. Small. T. D. Beacham, and K. J. Supemault. 



2001. Population structure of Fraser River chinook salmon 

 (Oncorhynchus tshawytscha): an analysis using microsatel- 

 lite DNA markers. Fish. Bull. 99:94-107. 



Olsen, J. B., P. Bentzen, and J. E. Seeb. 



1998. Characterization of seven microsatellite loci derived 

 from pink salmon. Mol. Ecol. 7: 1083-1090. 



