White et al.: Reproductive seasonality, fecundity, and spawning frequency of Toutoga onills 



429 











i 



fift^ 



fe^/^L.'^'. 



Figure 2, E-H. 



Histological stages of tautog ovaries (continued). (E) running ripe (stage 5), frame 1 shows 

 obvious lumen as distinguishing character, frame 2 shows typical field of view without lumen. 

 Hydrated oocytes have been ovulated (OHO) and now reside in the lumen (L). (F) partially 

 spent/redeveloping (stage 3a). (G) spent (stage 6). (H) resting (stage 7). PG = primary growth 

 oocyte. CA = cortical alveoli oocyte. AY = advanced yolked oocyte. GVM = germinal vesicle migra- 

 tion oocyte. GVBD = germinal vesicle breakdown oocyte. POF = postovulatory follicle. AO = 

 atretic oocyte. MA = macrophage aggregate. Magnification = 50x. 



stage oocytes, and a 5-mL aliquot was removed and placed 

 in a gridded petri dish. Grids were selected for counting 

 by using a random number table, and maximum diam- 

 eters of the first 500 oocytes encountered were measured 

 to the nearest 0.001 mm with a Biosonic Optical Pattern 

 Recognition System.® Data were grouped in 0.05-mm size 



classes for presentation (0.1 mm group=0.075 to 0.124 mm 

 oocytes). 



Batch fecundity was determined gravimetrically by 

 using a modification of the hydrated oocyte method (Hunt- 

 er etal., 1985). The method calls for both ovaries to be fixed 

 in IC/f formalin, but we had a formalin wet weight for only 



