NOTE Cordes and Graves: Hyridization and stock structure in Cynosaon regalis 



445 



1992).Type-I error was controlled 

 for all multiple testing with the 

 sequential Bonferroni method of 

 Rice (1989). 



Results and discussion 



Inclusion of nontarget 

 species in weakfish samples 



Initial analysis of the SOC050 mi- 

 crosatellite data revealed a sig- 

 nificant departure of genotypic 

 frequencies from expectations 

 of Hardy-Weinberg equilibrium 

 for the Georgia 1997 sample, 

 even after correction for mul- 

 tiple tests (a=0.005). Similarly, 

 initial SOC050 AMOVA results 

 indicated a significant within- 

 population variance (P=0.031), 

 and exact F permutation tests of 

 population pairwise F^,^ values 

 resulted in a number of near- 

 significant corrected P values, 

 all involving the Georgia 1997 

 sample. Inspection of the Georgia 

 1997 SOC050 alleles revealed a 

 bimodal size distribution due to 

 the presence of several unusually 

 small alleles less than 187 bp in 

 size. It was suspected that alleles 

 in the smaller mode might be the 

 result of misidentified individuals, 

 hybridization, or introgression. 



Analysis of putative weakfish 

 with small SOC0.50 alleles with 

 the 12S/16S marker of Cordes 

 et al. (2001) resulted in three 

 distinct restriction digestion 

 patterns. One pattern matched 

 that reported for weakfish, and 

 the other two did not match any 

 of the 16 species surveyed by 

 Cordes et al. ( 2001 ). To determine 

 the identity of the unknown pat- 

 terns, voucher samples of five ad- 

 ditional sciaenid species (listed 

 in the "Materials and methods" 

 section above) were analyzed 

 with the 12S/16S mitochondrial 

 marker. The two unknown pat- 

 terns matched those of silver 

 seatrout (Cynoscion nothus) and 

 sand seatrout (C arenarius) 

 (Table 2). The SOC050 locus was 

 subsequently amplified for all 

 silver seatrout (/! = 13) and sand 

 seatrout («=15) samples, produ- 



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