1 MARVIN ALLUM 



2 " Oyster Larvae Studies -- (Summary of Mr. 



3 Allum's Discussion). 



4 "The Project conducted two investigations 



5 using the larvae of the Pacific oyster as the test organisms. 



6 One was a field-sample study to measure the response of 



7 oyster larva to surface-water samples collected throughout 



8 each of the four study areas, and the other was a waste- 



9 sample study to determine the effects of the various waste 



10 streams of the pulp and paper mills on the larvae. 



11 "The flow diagram, following, shows the basic 



12 features of the field-sample response study. The series of 



13 boxes to the left illustrates the procedures used to obtain 



14 fertilized eggs; the series to the right deals with the col- 



15 lection of field samples and the initial analyses for 



16 chlorophyll, salinity, and SWL; and the lower center series 



17 shows the steps in the bioassay test, the measurements of 



18 responses, and the terminal chemical analyses. 



19 "Bioassay results were reported in terms of 



20 j the percent of abnormal larvae in each culture after incu- 



21 : bation. Larvae not fully shelled were counted as abnormal, 



22 I whereas all fully-shelled larvae were considered to be 



23 : normal, without regard to other abnormalities that might 

 i 



24 have been evident. This procedure avoided subjective 



25 interpretation in counting and provided a conservative 



